河南科技大学学报（医学版）
河南科技大學學報（醫學版）
하남과기대학학보（의학판）
Journal of Henan University of Science & Technology (Medical Science)
2015年
3期
161-163
,共3页
杨晖%和素娜%李杰%段冷昕%李艳%王建刚
楊暉%和素娜%李傑%段冷昕%李豔%王建剛
양휘%화소나%리걸%단랭흔%리염%왕건강
HPLC%山茱萸%果核%有机酸
HPLC%山茱萸%果覈%有機痠
HPLC%산수유%과핵%유궤산
high performance liguid chromatography%Cornus officinalis%kernel%organic acids
目的 建立HPLC法测定山茱萸果核中5种有机酸(草酸、酒石酸、DL-苹果酸、柠檬酸和没食子酸)含量的方法. 方法 采用Kromasil C18 (250 mm × 4. 6 mm i. d. , 5 μmm)色谱柱,3% CH3 OH 0. 01 mol·L-1 KH2 PO4 (pH 3. 0)溶液做流动相,流速0. 5 mL·min-1,柱温:25 ℃,检测波长210 nm. 结果 在32 min内实现了5种有机酸的基线分离,各有机酸测定的精密度为0. 29% ~1. 41%(n=6),回收率为89. 6% ~102. 0%. 结论 所建立的方法简便、准确、重现性好,可用于山茱萸果核中5种有机酸含量的测定.
目的 建立HPLC法測定山茱萸果覈中5種有機痠(草痠、酒石痠、DL-蘋果痠、檸檬痠和沒食子痠)含量的方法. 方法 採用Kromasil C18 (250 mm × 4. 6 mm i. d. , 5 μmm)色譜柱,3% CH3 OH 0. 01 mol·L-1 KH2 PO4 (pH 3. 0)溶液做流動相,流速0. 5 mL·min-1,柱溫:25 ℃,檢測波長210 nm. 結果 在32 min內實現瞭5種有機痠的基線分離,各有機痠測定的精密度為0. 29% ~1. 41%(n=6),迴收率為89. 6% ~102. 0%. 結論 所建立的方法簡便、準確、重現性好,可用于山茱萸果覈中5種有機痠含量的測定.
목적 건립HPLC법측정산수유과핵중5충유궤산(초산、주석산、DL-평과산、저몽산화몰식자산)함량적방법. 방법 채용Kromasil C18 (250 mm × 4. 6 mm i. d. , 5 μmm)색보주,3% CH3 OH 0. 01 mol·L-1 KH2 PO4 (pH 3. 0)용액주류동상,류속0. 5 mL·min-1,주온:25 ℃,검측파장210 nm. 결과 재32 min내실현료5충유궤산적기선분리,각유궤산측정적정밀도위0. 29% ~1. 41%(n=6),회수솔위89. 6% ~102. 0%. 결론 소건립적방법간편、준학、중현성호,가용우산수유과핵중5충유궤산함량적측정.
Objective To establish a quantitative HPLC method for determining five kinds of organic acids(oxalic acid, tartaric acid, DL-malic acid, citric acid and gallic acid) in cornus officinalis kernels. Methods A Kromasil C18 column(250 mm × 4. 6 mm i. d. , 5 μmm) was used with 3% CH3OH-0. 01 mol·L-1 KH2PO4(pH 3. 0)as the mobile phase at a flow rate of 0. 5 mL·min-1. The column temperature was 25℃ and the UV detection wavelength was 210 nm. Results The five organic acids were separated in 32 min. The average recoveries of the method were 89. 6% ~102. 0% with the relative standard deviation of 0. 29% ~1. 41%(n=6). Conclusion The method is simple, accurate, reproducible and practical for the detection of organic acids in cornus officinalis kernels.
