CAJ | 학술논문

目的：建立脐血单个核细胞与 K562细胞共培养体系，探讨脐血单个核细胞对 K562细胞增殖的影响。方法将脐血单个核细胞和对数生长期的 K562细胞采用 Transwell 小室共培养，以单独培养的 K562细胞为对照，于培养后的第1、3、5、7天分别选择6孔细胞进行计数比较。结果培养后第3、5、7天，共培养组 K562细胞较单独培养的 K562细胞明显增加，差异均有统计学意义（P ＜0．01）；随着培养时间的延长，组间差异增大。结论脐血单个核细胞可以促进 K562细胞的增殖。
목적：건립제혈단개핵세포여 K562세포공배양체계，탐토제혈단개핵세포대 K562세포증식적영향。방법장제혈단개핵세포화대수생장기적 K562세포채용 Transwell 소실공배양，이단독배양적 K562세포위대조，우배양후적제1、3、5、7천분별선택6공세포진행계수비교。결과배양후제3、5、7천，공배양조 K562세포교단독배양적 K562세포명현증가，차이균유통계학의의（P ＜0．01）；수착배양시간적연장，조간차이증대。결론제혈단개핵세포가이촉진 K562세포적증식。
Objective To explore the effects of umbilical cord blood mononuclear cells on the proliferation of K562 cells using a co -culture system of both cells.Methods The separated umbilical cord blood mononuclear cells and K562 cells at the logarithmic phase were co -cultured in Transwell chambers, while individually cultured K562 cells were taken as the control.Then, the number of K562 cells within six wells was counted on Days 1, 3, 5, and 7 days of culti-vation.Results According to the cell growth curve, the number of K562 cells in the co -cultured group was much high-er than that in the control group on Days 3, 5 and 7 of cultivation (P <0.05).The inter -group difference was increased as the extension of incubation time.Conclusion Umbilical cord blood mononuclear cells can stimulate the proliferation of K562 cells.