CAJ | 학술논문

目的：研究酪氨酸216位点磷酸化糖原合成酶激酶－3β〔p －GSK －3β（Try216）〕在肥胖多囊卵巢综合征（PCOS）患者卵巢颗粒细胞中的表达。方法选择因 PCOS、不孕行体外受精／单精子卵胞浆内注射－胚胎移植（IVF／ICSI －ET）取卵的患者10例，其中 PCOS 肥胖组〔体质指数（BMI）≥28．0 kg／m2〕5例，PCOS 非肥胖组（18．5 kg／m 2＜BMI≤23．9 kg／m 2）5例；另外选择因输卵管梗阻或男方因素行 IVF／ICSI －ET 取卵者5例作为非PCOS 对照组（18．5 kg／m 2＜BMI≤23．9 kg／m 2）。取卵后收集颗粒细胞进行培养，葡萄糖氧化酶（GOD）法检测第48 h 细胞培养液葡糖糖含量，免疫印迹法检测 p －GSK －3β（Try216）表达。结果非 PCOS 对照组、PCOS 非肥胖组、PCOS 肥胖组中葡萄糖含量依次增高（P ＜0．05），p －GSK －3β（Try216）表达量依次增加（P ＜0．05），差异均有统计学意义。结论PCOS 患者卵巢颗粒细胞中糖代谢异常，肥胖加重 PCOS 患者糖代谢紊乱。 p －GSK －3β（Try216）在肥胖 PCOS 患者糖代谢紊乱中发挥重要作用。
목적：연구락안산216위점린산화당원합성매격매－3β〔p －GSK －3β（Try216）〕재비반다낭란소종합정（PCOS）환자란소과립세포중적표체。방법선택인 PCOS、불잉행체외수정／단정자란포장내주사－배태이식（IVF／ICSI －ET）취란적환자10례，기중 PCOS 비반조〔체질지수（BMI）≥28．0 kg／m2〕5례，PCOS 비비반조（18．5 kg／m 2＜BMI≤23．9 kg／m 2）5례；령외선택인수란관경조혹남방인소행 IVF／ICSI －ET 취란자5례작위비PCOS 대조조（18．5 kg／m 2＜BMI≤23．9 kg／m 2）。취란후수집과립세포진행배양，포도당양화매（GOD）법검측제48 h 세포배양액포당당함량，면역인적법검측 p －GSK －3β（Try216）표체。결과비 PCOS 대조조、PCOS 비비반조、PCOS 비반조중포도당함량의차증고（P ＜0．05），p －GSK －3β（Try216）표체량의차증가（P ＜0．05），차이균유통계학의의。결론PCOS 환자란소과립세포중당대사이상，비반가중 PCOS 환자당대사문란。 p －GSK －3β（Try216）재비반 PCOS 환자당대사문란중발휘중요작용。
Objective To investigate the level of p -GSK -3β(Try216) in the ovarian granulosa cells of obese pa-tients with polycystic ovarian syndrome (PCOS).Methods A total of ten patients were enrolled into the current study who underwent in vitro fertilization/intracytoplasmic sperm injection and embyro transfer (IVF/ICSI -ET) due to infertil-ity caused by PCOS.These patients were divided into two groups according to their conditions: an obese PCOS group (BMI≥28 kg/m 2 , n =5) and a non -obese PCOS group (18.5 kg/m 2 <BMI≤23.9 kg/m 2 , n =5).Meanwhile, an-other five patients were adopted as a non -PCOS control group (18.5 kg/m2 <BMI≤23.9 kg/m2 ) who suffered from blocked fallopian tubes or underwent IVF/ICSI -ET due to male infertility.Granulosa cells were collected from patients during IVF/ICSI -ET.The production of glucose in the culture medium was measured by GOD method after 48 h of culti-vation.The quantity of p -GSK -3β(Try216) in the ovarian granulosa cells was detected by Western blotting analysis. Results The levels of glucose and p -GSK -3β(Try216) was highest in the obese PCOS group, followed by the non -obese PCOS group and then the non -PCOS group (P <0.05).Conclusion Abnormal glucose metabolism is present in the ovarian granulosa cells of PCOS patients, which can be aggravated by obesity.The protein p -GSK -3β(Try216) is essential for the glucose metabolism of obese patients with PCOS.