CAJ | 학술논문

目的：研究α－细辛醚对大鼠星型胶质细胞活化的影响。方法诱发大鼠癫痫持续状态（SE）1 h，用胶质纤维酸性蛋白（GFAP）免疫组化染色观察正常组、模型组和实验组的大鼠海马区的星型胶质细胞活化情况；原代大鼠海马星型胶质细胞传代后，分为正常组、脂多糖诱导活化模型组和实验组；用CCK－8试剂盒检测各组细胞的增殖情况，用Western －blotting 法检测各组 GFAP表达。结果模型组在SE后第1天的海马星型胶质细胞活化最明显，在第28天恢复至正常水平；实验组的星型胶质细胞活化水平在第1天、第3天和第7天均低于模型组（ P＜0.05）。高、低2个剂量（50，100μg · mL－1）α－细辛醚对体外脂多糖诱导的星型胶质细胞的增殖有明显抑制作用（ P＜0.05）；高浓度实验组对脂多糖诱导星型胶质细胞的 GFAP 表达增多有明显抑制作用（ P ＜0.05）。结论α－细辛醚对大鼠海马星型胶质细胞的活化有抑制作用。
목적：연구α－세신미대대서성형효질세포활화적영향。방법유발대서전간지속상태（SE）1 h，용효질섬유산성단백（GFAP）면역조화염색관찰정상조、모형조화실험조적대서해마구적성형효질세포활화정황；원대대서해마성형효질세포전대후，분위정상조、지다당유도활화모형조화실험조；용CCK－8시제합검측각조세포적증식정황，용Western －blotting 법검측각조 GFAP표체。결과모형조재SE후제1천적해마성형효질세포활화최명현，재제28천회복지정상수평；실험조적성형효질세포활화수평재제1천、제3천화제7천균저우모형조（ P＜0.05）。고、저2개제량（50，100μg · mL－1）α－세신미대체외지다당유도적성형효질세포적증식유명현억제작용（ P＜0.05）；고농도실험조대지다당유도성형효질세포적 GFAP 표체증다유명현억제작용（ P ＜0.05）。결론α－세신미대대서해마성형효질세포적활화유억제작용。
Objective To investigate the effects of α-asarone on acti-vation of astrocytes of rat hippocampus.Methods Rat status epilepticus ( SE) lasted for 1 hour.The activations of astrocyte in rat hippocampus of control group , model group and α-asarone treatment group were ob-served by Glial fibrillary acidic protein ( GFAP) immunohistochemistry.The astrocytes of rat hippocampus after passage were randomly assigned to control group , lipopolysaccharide ( LPS ) model group and α-asarone treatment group.Then the ability of cell proliferation were detected by CCK8.The GFAP were detected by Western -blotting .Results The most obvious activation of astrocyte in hippocampus of post -SE rats was observed at 1 d, then back to normal at 28 d after SE.Compared with model group , the activated levels of astrocyte in α-asarone treatment group were significantly decreased at 1,3,7 days after SE (P<0.05).Compared with model group , proliferation of astrocyte induced by LPS were significantly decreased in 50 μg· mL-1 and 100 μg· mL-1 ofα-asarone treatment groups and GFAP expression were significantly de-creased in 100 μg· mL-1α-asarone group (P<0.05).Conclusionα -Asarone could decreased the activation of astrocyte of rat hippocampus.