CAJ | 학술논문

目的：研究载脂蛋白E基因多态性对丹参素调脂作用的影响。方法用逆转录聚合酶链反应法检测不同基因型载脂蛋白E与丹参素共同作用于L－02细胞后，L－02细胞低密度脂蛋白受体和羟甲戊二酰辅酶A还原酶mRNA表达的变化。结果 ApoE 能够明显减弱丹参素对 LDLR mRNA 表达的上调（ P＜0.01），突变型E2（ Arg112 Cys ）减弱强度明显大于野生型E3和突变型E4（ Cys 158 Arg，P＜0.01）；ApoE亦能够增强丹参素对羟甲戊二酰辅酶A还原酶mRNA表达的下调， E4增强作用最大（ P ＜0.01），但 E2、E3作用不明显（ P＞0.05）。结论载脂蛋白E 112和158位均可能是影响丹参素调脂作用的关键位点。
목적：연구재지단백E기인다태성대단삼소조지작용적영향。방법용역전록취합매련반응법검측불동기인형재지단백E여단삼소공동작용우L－02세포후，L－02세포저밀도지단백수체화간갑무이선보매A환원매mRNA표체적변화。결과 ApoE 능구명현감약단삼소대 LDLR mRNA 표체적상조（ P＜0.01），돌변형E2（ Arg112 Cys ）감약강도명현대우야생형E3화돌변형E4（ Cys 158 Arg，P＜0.01）；ApoE역능구증강단삼소대간갑무이선보매A환원매mRNA표체적하조， E4증강작용최대（ P ＜0.01），단 E2、E3작용불명현（ P＞0.05）。결론재지단백E 112화158위균가능시영향단삼소조지작용적관건위점。
Objective To study the action of apolipoprotein E genetic polymorphism on the efficacy of Tanshinol.Methods Through detecting the expression of low density lipoprotein receptor mRNA and 3-hydroxy-3 -methylglutaryl coenzyme A reductase mRNA in L -02 cells by reverse transcription-polymerase chain reaction , to compare the effect of different genetic subtypes of ApoE on Tanshinol.Results Apolipopro-tein E can weaken the induction of low density lipoprotein receptor ex-pression caused by Tanshinol.The weakening extent of apolipoprotein E 2 (Arg112Cys)was stronger than apolipoprotein E3 and apolipoprotein E4 ( Cys 158 Arg) ( P<0.01 ).Apolipoprotein E also can enhance the inhi-bition of 3-hydroxy -3-methylglutaryl coenzyme A reductase expres-sion, especially apolipoprotein E4 (P<0.01).But apolipoprotein E2 or apolipoprotein E3 can not have statistical significance ( P >0.05 ).Conclusion Both 112 and 158 site might be the key site that have effect on the efficacy of Tanshinol.