中国临床药理学杂志
中國臨床藥理學雜誌
중국림상약이학잡지
The Chinese Journal of Clinical Pharmacology
2015年
17期
1749-1753
,共5页
莲子心萃取物%肝纤维化%肝星状细胞%肿瘤坏死因子-a%转化生长因子β1
蓮子心萃取物%肝纖維化%肝星狀細胞%腫瘤壞死因子-a%轉化生長因子β1
련자심췌취물%간섬유화%간성상세포%종류배사인자-a%전화생장인자β1
Plumula Nelumbinis extract%hepatic fibrosis%hepatic stellate cell%tumor necrosis factor-α%transforming growth factor-β1
目的:以体外和动物模型探讨莲子心萃取物对于肝星状细胞活化及大鼠肝纤维化的影响。方法在细胞实验中,检测莲子心萃取物对肝星状细胞珠的影响,用荧光分析仪测定细胞内过氧化氢含量,用Western blot检测胶原蛋白沉积、核转录因子-κB( NF-κB)的活化、Iκba的磷酸化与甲型平滑肌动蛋白的表达。动物分3组:假手术组,给予0.7%的羧甲基纤维素( CMC );模型组,结扎大鼠胆管,并给予0.7%CMC;实验组,结扎大鼠胆管并给予0.5 g · kg-1莲子心萃取物,每天喂药2次。用比色分析仪检测莲子心萃取物对大鼠谷草转氨酶(AST)和谷丙转氨酶(ALT)的含量,用定量实时聚合酶反应检测肝纤维化分数、甲型平滑肌动蛋白α-SMA及胶原蛋白col1α2的基因表达的影响。结果与空白组比较,80μg· mL-1莲子心萃取物可抑制TNF-α所引发的肝星状细胞内的过氧化氢含量与甲型平滑肌动蛋白(α-SMA)蛋白质表达及细胞内核转录因子抑制蛋白α( IκBα)的磷酸化,并抑制核转录因子( NF-κB)的活化及α-SMA蛋白质的表达;80μg· mL-1莲子心萃取物可显著抑制细胞胶原蛋白生成。与模型组比较,莲子心萃取物可降低肝纤维化大鼠的AST和ALT含量,降低肝胶原蛋白含量,减少α-SMA、犬Ⅰ型胶原α2( col1α2) mRNA表达。结论莲子心萃取物能抑制肝纤维化。
目的:以體外和動物模型探討蓮子心萃取物對于肝星狀細胞活化及大鼠肝纖維化的影響。方法在細胞實驗中,檢測蓮子心萃取物對肝星狀細胞珠的影響,用熒光分析儀測定細胞內過氧化氫含量,用Western blot檢測膠原蛋白沉積、覈轉錄因子-κB( NF-κB)的活化、Iκba的燐痠化與甲型平滑肌動蛋白的錶達。動物分3組:假手術組,給予0.7%的羧甲基纖維素( CMC );模型組,結扎大鼠膽管,併給予0.7%CMC;實驗組,結扎大鼠膽管併給予0.5 g · kg-1蓮子心萃取物,每天餵藥2次。用比色分析儀檢測蓮子心萃取物對大鼠穀草轉氨酶(AST)和穀丙轉氨酶(ALT)的含量,用定量實時聚閤酶反應檢測肝纖維化分數、甲型平滑肌動蛋白α-SMA及膠原蛋白col1α2的基因錶達的影響。結果與空白組比較,80μg· mL-1蓮子心萃取物可抑製TNF-α所引髮的肝星狀細胞內的過氧化氫含量與甲型平滑肌動蛋白(α-SMA)蛋白質錶達及細胞內覈轉錄因子抑製蛋白α( IκBα)的燐痠化,併抑製覈轉錄因子( NF-κB)的活化及α-SMA蛋白質的錶達;80μg· mL-1蓮子心萃取物可顯著抑製細胞膠原蛋白生成。與模型組比較,蓮子心萃取物可降低肝纖維化大鼠的AST和ALT含量,降低肝膠原蛋白含量,減少α-SMA、犬Ⅰ型膠原α2( col1α2) mRNA錶達。結論蓮子心萃取物能抑製肝纖維化。
목적:이체외화동물모형탐토련자심췌취물대우간성상세포활화급대서간섬유화적영향。방법재세포실험중,검측련자심췌취물대간성상세포주적영향,용형광분석의측정세포내과양화경함량,용Western blot검측효원단백침적、핵전록인자-κB( NF-κB)적활화、Iκba적린산화여갑형평활기동단백적표체。동물분3조:가수술조,급여0.7%적최갑기섬유소( CMC );모형조,결찰대서담관,병급여0.7%CMC;실험조,결찰대서담관병급여0.5 g · kg-1련자심췌취물,매천위약2차。용비색분석의검측련자심췌취물대대서곡초전안매(AST)화곡병전안매(ALT)적함량,용정량실시취합매반응검측간섬유화분수、갑형평활기동단백α-SMA급효원단백col1α2적기인표체적영향。결과여공백조비교,80μg· mL-1련자심췌취물가억제TNF-α소인발적간성상세포내적과양화경함량여갑형평활기동단백(α-SMA)단백질표체급세포내핵전록인자억제단백α( IκBα)적린산화,병억제핵전록인자( NF-κB)적활화급α-SMA단백질적표체;80μg· mL-1련자심췌취물가현저억제세포효원단백생성。여모형조비교,련자심췌취물가강저간섬유화대서적AST화ALT함량,강저간효원단백함량,감소α-SMA、견Ⅰ형효원α2( col1α2) mRNA표체。결론련자심췌취물능억제간섬유화。
Objective To study the effect of Plumula Nelumbinis extract ( PNE) on hepatic stellate cell activation and liver fibrosis in vitro and in vivo in rats model.Methods PNE impact on hepatic stellate cells was detected by cell experiments . The hydrogen peroxide content was determined in the cell by fluorescence analyzer.The ollagen deposition , nuclear factor -κB ( NF -κB ) activation , Iκbαphosphorylation ,α-SMA protein expression and gene transcription were detected by Western blot.Animals are divided into three groups , sham group , 0.7%carboxymethyl cellulose ( CMC ) was administrated; model group , Bile duct ligation in rats , 0.7%CMC was administrated;experimental group , Bile duct ligation in rats and PNE was administrated , twice a day from 3 th week.PNE impact on aspartate transaminase ( AST ) and alanine transaminase ( ALT ) was detected by colorimetric analyzer.The liver fibrosis scores,α-SMA and col1 α2 expression was detected by quanti-tative real -time polymerase chain reaction.Results Compared with blank group , PNE (80 μg· mL-1 ) can inhibit intracellular hydrogen peroxide content of hepatic stellate cells caused by TNF -α, alpha smooth muscle actin (α-SMA) protein expression, and intracellular NF-kappa B inhibitor-α(IκBα) phosphoryla-tion, and inhibit NF-κB activation and α-SMA protein expression.PNE (80 μg· mL-1 ) can significantly inhibit cell collagen production.In the experiment where Bile duct ligation ( BDL ) induced liver fibrosis in rats.Compared with model group,the use of PNE can reduce AST and ALT , decrease hepatic collagen content , and reduce α-SMA, mouse collagen type I Alpha 2 ( col1α2 ) mRNA expression.Conclusion PNE can inhibit liver fibrosis.