CAJ | 학술논문

目的：研究水黄皮胶囊剂的抗血栓作用并从抗凝、纤溶两个方面初步探讨其作用机制。方法制备小鼠断尾出血、小鼠尾血栓、大鼠下腔静脉血栓3种模型，小鼠随机分为5组（每组8只）：空白组（0.9％NaCl ）或模型组（0.5％CMC－Na）、阳性对照组（阿司匹林100 mg· kg－1）、水黄皮胶囊剂高、中、低3个剂量组（900，300，100 mg· kg－1）。大鼠随机分为6组（每组8只）：假手术组（0.9％NaCl）、模型组（0.5％CMC －Na ）、阳性对照组（阿司匹林50 mg· kg－1）、水黄皮胶囊剂高、中、低3个剂量组（450，150，50 mg· kg－1），每天灌胃1次，连续15 d。光镜下观察大鼠下腔静脉的病理变化，并测定大鼠血浆凝血酶原时间（ PT）、活化部分凝血活酶时间（ APTT）以及组织型纤溶酶原激活剂（t－PA）和纤溶酶原激活物抑制因子1（PAI－1）水平。结果水黄皮胶囊剂能明显延长PT和APTT时间，升高血浆t－PA 活性、降低PAI－1活性。结论水黄皮胶囊剂有显著抗血栓作用，其作用机制可能与抗凝系统和纤溶系统活性升高有关。
목적：연구수황피효낭제적항혈전작용병종항응、섬용량개방면초보탐토기작용궤제。방법제비소서단미출혈、소서미혈전、대서하강정맥혈전3충모형，소서수궤분위5조（매조8지）：공백조（0.9％NaCl ）혹모형조（0.5％CMC－Na）、양성대조조（아사필림100 mg· kg－1）、수황피효낭제고、중、저3개제량조（900，300，100 mg· kg－1）。대서수궤분위6조（매조8지）：가수술조（0.9％NaCl）、모형조（0.5％CMC －Na ）、양성대조조（아사필림50 mg· kg－1）、수황피효낭제고、중、저3개제량조（450，150，50 mg· kg－1），매천관위1차，련속15 d。광경하관찰대서하강정맥적병리변화，병측정대서혈장응혈매원시간（ PT）、활화부분응혈활매시간（ APTT）이급조직형섬용매원격활제（t－PA）화섬용매원격활물억제인자1（PAI－1）수평。결과수황피효낭제능명현연장PT화APTT시간，승고혈장t－PA 활성、강저PAI－1활성。결론수황피효낭제유현저항혈전작용，기작용궤제가능여항응계통화섬용계통활성승고유관。
Objective To study the antithrombotic effects of Pongamia pinnata capsules and to explore its mechanism from anticoagulat action and fibrinolysis.Methods Three experimental models , including mouse tail bleeding model , mouse tail thrombosis model and inferior vena cava thrombosis model were established.The mice were randomly divided into 5 groups(8 mice per group):blank or model group (0.5%CMC-Na), positive control group ( aspirin, 100 mg · kg -1 ) , Pongamia pinnata capsules of the high dose group , middle dose group and low dose group (in accordance with 900,300,100 mg· kg -1 ).The SD rats were ran-domly divided into 6 groups ( 8 mice per group ):sham group ( 0.9%NaCl), model group(0.5%CMC-Na), positive control group(aspirin, 50 mg· kg-1), Pongamia pinnata capsules of the high dose group , middle dose group and low dose group ( in accordance with 450 ,150 ,50 mg· kg -1 ), gavage once daily for 15 d.The pathological changes of inferior vena cava tissue were observed by light microscope.The pro-thrombin time ( PT),activated partial thromboplastin time ( APTT) and the contents of tissue type plasminogen activator ( t -PA ) and plasminogen activator inhibitor -1 ( PAI -1 ) were measured .Results The PT and APTT were significantly prolonged.The release of t -PA was promoted and the release of PAI -1 was inhibited. Conclusion There is a significant antithrombotic effects about Pongamia pinnata capsules and its mechanism may be related to increasing the activity of the anticoagulant factor and fibrinolysis system.