CAJ | 학술논문

目的：检测并对比维吾尔族甲状腺癌与甲状腺良性结节患者、维吾尔族甲状腺癌与汉族甲状腺癌患者血清蛋白质指纹图谱，筛选不同种族特异的甲状腺癌蛋白质标记物。方法运用二维聚丙烯酰胺凝胶电泳(2D-PAGE)技术，构建维吾尔族、汉族甲状腺癌与维吾尔族甲状腺良性结节的蛋白表达谱，通过图像分析软件交叉比较两组样本，运用液相色谱-质谱/质谱联用( LC-MS/MS)技术对差异蛋白进行鉴定，并在Swissport数据库中搜索蛋白名称、细胞定位及功能分类。结果维吾尔族甲状腺良性结节与甲状腺癌患者血清对比有11种蛋白质差异表达，其中补体C3、C4b在维吾尔族甲状腺良性结节患者血清中下调表达，血红素结合蛋白等9种蛋白质上调表达。维吾尔、汉族甲状腺癌患者血清对比有7种蛋白质差异表达，其中转铁蛋白在维吾尔族甲状腺癌患者血清中上调表达，细胞角蛋白-1等6种蛋白质下调表达。结论血红素结合蛋白、α2-巨球蛋白、转铁蛋白等多种标记蛋白联合筛检可为维吾尔、汉族甲状腺癌的诊断提供依据。
목적：검측병대비유오이족갑상선암여갑상선량성결절환자、유오이족갑상선암여한족갑상선암환자혈청단백질지문도보，사선불동충족특이적갑상선암단백질표기물。방법운용이유취병희선알응효전영(2D-PAGE)기술，구건유오이족、한족갑상선암여유오이족갑상선량성결절적단백표체보，통과도상분석연건교차비교량조양본，운용액상색보-질보/질보련용( LC-MS/MS)기술대차이단백진행감정，병재Swissport수거고중수색단백명칭、세포정위급공능분류。결과유오이족갑상선량성결절여갑상선암환자혈청대비유11충단백질차이표체，기중보체C3、C4b재유오이족갑상선량성결절환자혈청중하조표체，혈홍소결합단백등9충단백질상조표체。유오이、한족갑상선암환자혈청대비유7충단백질차이표체，기중전철단백재유오이족갑상선암환자혈청중상조표체，세포각단백-1등6충단백질하조표체。결론혈홍소결합단백、α2-거구단백、전철단백등다충표기단백연합사검가위유오이、한족갑상선암적진단제공의거。
Objective To compare serum protein fingerprint among Uygur patients with thyroid cancer and benign thyroid nodules, and Han patients with thyroid cancer, and to screen ethnic-specific protein markers of thyroid cancer. Methods Using the technology of two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), the protein expression profiles of Uygur and Han patients with thyroid cancer and Uygur patients with thyroid nodules were established and compared by image analysis software between two groups. Liquid chromatography-mass spectrometry/mass spectrometry coupling techniques ( LC-MS/MS) were used to identify differential protein. The protein′s name, cellular localization and functional classification were searched in Swissport database. Results There are 11 differentially expressed proteins by comparison of sera in Uygur patients with benign thyroid nodules and thyroid cancer, in which complement C3 and C4b levels were down-regulated in the serums of Uygur patients with benign thyroid nodules, and 9 proteins such as heme-binding proteins etc were up-regulated. There are 7 differentially expressed proteins by comparison of serums in Uygur and Han patients with thyroid cancer, in which transferrin level was up-regulated in the serum of Uygur patients with thyroid cancer, and 6 proteins such as cytokeratin-1 etc were down-regulated in serum of Uygur patients with thyroid cancer. Conclusion Combined screening of multiple labelled proteins including heme-binding protein,α2-macroglobulin, and transferrin protein etc may provide the basis for the diagnosis of thyroid cancer in Uygurs and Hans.