CAJ | 학술논문

目的：探讨肥大细胞活化相关抗体及分子IgE、抗IgE、FcεRⅠα、抗FcεRⅠ在自身免疫性肝病患者血清中的表达水平及其与该病的关系。方法病例对照研究。收集上海市第一人民医院消化科2014年5月至11月收治的77例血清肝病相关抗体谱检测阳性患者血清标本及临床资料，其中自身免疫性肝病组33例，病毒性肝炎等其他肝脏疾病组44例；健康体检者64例作健康对照组。采用ELISA法检测血清标本肥大细胞相关的抗IgE、FcεRⅠα、抗FcεRⅠ；速率散射比浊法检测血清IgE、IgM、IgG水平。比较自身免疫性肝病组、其他肝脏疾病组与健康对照组间IgE、抗IgE、FcεRⅠα、抗FcεRⅠ水平的差异。检测结果组间比较采用Mann－Whitney U秩和检验。结果肝病相关自身抗体检测阳性患者血清抗IgE、FcεRⅠα、抗FcεRⅠ水平分别为1．74（1．16～2．88）mg／L、14．86（4．39～26．23） mg／L和47．22（36．89～55．29） mg／L 均明显高于健康对照组1．23（0．95～1．58） mg／L、1．87（1．52～2．33）mg／L及35．40（24．74～44．89）mg／L，差异有统计学意义（U＝1614，556．5，1319．5；P均＜0．01）。其他肝脏疾病组FcεRⅠα分子水平为18．40（7．35～30．64） mg／L，高于自身免疫性肝病组6．25（2．49～22．29）mg／L，差异有统计学意义（U＝445，P＜0．01）。结论血清肝病相关抗体谱检测阳性患者血清中肥大细胞相关的抗IgE、FcεRⅠα、抗FcεRⅠ水平表达升高。 AILD患者较其他肝病者血清FcεRⅠα水平低。肥大细胞相关的抗IgE、FcεRⅠα及抗FcεRⅠ分子参与肝脏疾病的炎症损伤。（中华检验医学杂志，2015，38：617－621）
목적：탐토비대세포활화상관항체급분자IgE、항IgE、FcεRⅠα、항FcεRⅠ재자신면역성간병환자혈청중적표체수평급기여해병적관계。방법병례대조연구。수집상해시제일인민의원소화과2014년5월지11월수치적77례혈청간병상관항체보검측양성환자혈청표본급림상자료，기중자신면역성간병조33례，병독성간염등기타간장질병조44례；건강체검자64례작건강대조조。채용ELISA법검측혈청표본비대세포상관적항IgE、FcεRⅠα、항FcεRⅠ；속솔산사비탁법검측혈청IgE、IgM、IgG수평。비교자신면역성간병조、기타간장질병조여건강대조조간IgE、항IgE、FcεRⅠα、항FcεRⅠ수평적차이。검측결과조간비교채용Mann－Whitney U질화검험。결과간병상관자신항체검측양성환자혈청항IgE、FcεRⅠα、항FcεRⅠ수평분별위1．74（1．16～2．88）mg／L、14．86（4．39～26．23） mg／L화47．22（36．89～55．29） mg／L 균명현고우건강대조조1．23（0．95～1．58） mg／L、1．87（1．52～2．33）mg／L급35．40（24．74～44．89）mg／L，차이유통계학의의（U＝1614，556．5，1319．5；P균＜0．01）。기타간장질병조FcεRⅠα분자수평위18．40（7．35～30．64） mg／L，고우자신면역성간병조6．25（2．49～22．29）mg／L，차이유통계학의의（U＝445，P＜0．01）。결론혈청간병상관항체보검측양성환자혈청중비대세포상관적항IgE、FcεRⅠα、항FcεRⅠ수평표체승고。 AILD환자교기타간병자혈청FcεRⅠα수평저。비대세포상관적항IgE、FcεRⅠα급항FcεRⅠ분자삼여간장질병적염증손상。（중화검험의학잡지，2015，38：617－621）
Objective To investigate the level and value of serum IgE, anti-IgE, FcεRⅠα, anti-FcεRⅠin autoimmune liver disease ( AILD) .Methods In this case-control study, the serum samples and clinical data of 77 patients with hepatosis were collected between May and November 2014 from the department of gastroenterology of Shanghai First People′s Hospital.These patients had positive results about the liver-related autoimmune antibodies, including 33 cases of AILD, 44 cases of other chronic liver disease. 64 healthy persons were collected as control group.Serum mast cell-associated anti-IgE, FcεRⅠα, anti-FcεRⅠwere detected by Enzyme-linked Immuno sorbent Assay ( ELISA) .Serum IgE, IgM and IgG were detected by rate scatter nephelometry.Differences among AILD, other chronic liver disease and healthy control were assessed.Results were compared using Mann-Whitney U test.Results Mast cell-associated anti-IgE, FcεRⅠα, anti-FcεRⅠin liver-related autoimmune antibodies positive patients were significantly higher than healthy control [1.74(1.16 -2.88)mg/L, 14.86(4.39 -26.23)mg/L, 47.22(36.89 -55.29)mg/L and 1.23(0.95-1.58)mg/L, 1.87(1.52-2.33)mg/L, 35.40(24.74-44.89)mg/L, respectively;U=1614,556.5,1319.5, P<0.01].FcεRⅠαwas significantly higher in other chronic liver disease patients than AILD patients [18.40(7.35-30.64)mg/L and 6.25(2.49-22.29), respectively;U=445, P<0.01] .Conclusion Mast cell-associated anti-IgE, FcεRⅠα, anti-FcεRⅠwere increased in liver-related autoimmune antibodies positive hepatosis patients.However, FcεRⅠαwas lower in AILD than other chronic liver disease.Mast cell-associated anti-IgE、FcεRⅠαand anti-FcεRⅠ molecules involved in the inflammatory lesion of liver disease.