CAJ | 학술논문

将同一批经雌核发育产生的F1彭泽鲫(Carassius auratus var. pengze)仔鱼(Pcc)分别置于实验室和池塘进行养殖,结果发现,与池塘养殖雄鱼比例极少相比,实验室养殖中出现了高比例的雄鱼。实验室养殖 PccF1雄雌比例为(43.6±3.0)%,而池塘养殖PccF1雌雄比为(4.7±1.2)%。本研究比较了不同养殖模式下雌雄鱼性腺分化相关基因的表达,结果发现除Pcc-vasa、Pcc-esr1和Pcc-esr2b外,实验室养殖PccF1精巢中性腺分化、受体、类固醇合成酶类基因表达极显著(P<0.01)或显著(P<0.05)高于卵巢中对应基因的表达量。对于池塘养殖的 PccF1,除 Pcc-amh、Pcc-dmrt1b、Pcc-dmrt1c、Pcc-foxl2、Pcc-vasa 和 Pcc-esr2b 外,精巢中性腺分化和受体基因表达极显著(P<0.01)或显著(P<0.05)高于卵巢中对应基因的表达量；但精巢中绝大部分类固醇合成酶类基因的表达量极显著低于卵巢(P<0.01)。实验室和池塘养殖 PccF1雌雄出现差异表达的基因主要是类固醇合成酶类及调控芳香化酶的转录因子,这些基因的差异表达可能与雌雄激素的合成或调控相关,从而导致不同养殖模式出现不同比例的雄鱼。
장동일비경자핵발육산생적F1팽택즉(Carassius auratus var. pengze)자어(Pcc)분별치우실험실화지당진행양식,결과발현,여지당양식웅어비례겁소상비,실험실양식중출현료고비례적웅어。실험실양식 PccF1웅자비례위(43.6±3.0)%,이지당양식PccF1자웅비위(4.7±1.2)%。본연구비교료불동양식모식하자웅어성선분화상관기인적표체,결과발현제Pcc-vasa、Pcc-esr1화Pcc-esr2b외,실험실양식PccF1정소중성선분화、수체、류고순합성매류기인표체겁현저(P<0.01)혹현저(P<0.05)고우란소중대응기인적표체량。대우지당양식적 PccF1,제 Pcc-amh、Pcc-dmrt1b、Pcc-dmrt1c、Pcc-foxl2、Pcc-vasa 화 Pcc-esr2b 외,정소중성선분화화수체기인표체겁현저(P<0.01)혹현저(P<0.05)고우란소중대응기인적표체량；단정소중절대부분류고순합성매류기인적표체량겁현저저우란소(P<0.01)。실험실화지당양식 PccF1자웅출현차이표체적기인주요시류고순합성매류급조공방향화매적전록인자,저사기인적차이표체가능여자웅격소적합성혹조공상관,종이도치불동양식모식출현불동비례적웅어。
Pengze crucian carp (Carassius auratus var. pengze, Pcc) is naturally gynogenic, and gynogenic Pcc pro-duced by artificial breeding can theoretically produce solely female offspring. In laboratory culture, a higher proportion of male fish occurred in F1 progenies compared with pond culture conditions. A higher proportion of males was found in the F1 progeny under laboratory culture (43.6%) compared with pond (4.7%) conditions. To determine the cause of this variant sex ratio, ovarian gene expression profiles were detected and compared between the male and female F1 progenies for different culture conditions. Results showed that expressions of most testicular sex differentiation-related, steroid receptor, and steroidogenic genes in PccF1 offspring were significantly higher than those in ovaries under labo-ratory culture conditions, except for Pcc-vasa, Pcc-esr1, and Pcc-esr2b. With respect to pond culture conditions, ex-pressions of most testicular sex differentiation-related and steroid receptor genes in PccF1 offspring were significantly higher than those in ovaries, except for Pcc-amh, Pcc-dmrt1b, Pcc-dmrt1c, Pcc-foxl2, Pcc-vasa, and Pcc-esr2b. Amounts of the steroidogenic genes had reversed expression patterns (male<female). The gene expression profiles for steroidogenesis and transcriptional regulation of aromatase were different in the gonads of F1 progenies between labo-ratory and pond culture conditions. The variant sex ratio may be attributed to the differential gene expression of both steroidogenic enzymes and transcription factors of aromatase under different culture densities, which may be associated with the regulation and/or synthesis of endogenous steroid hormone levels and related to different sex ratios.