CAJ | 학술논문

目的：建立异硫氰酸苯酯（ PITC）柱前衍生高效液相法测定糖尿病患者血浆和尿液中游离氨基酸含量。方法用Waters-e2695操作系统，PITC为柱前衍生试剂，色谱柱为Shim-pack VP-ODS（250 mm ×4.6 mm，5μm），流动相为甲醇-乙腈-水和醋酸钠溶液，用醋酸调pH至6.5，梯度洗脱，用紫外254 nm检测。结果该方法线性范围为2~200μg· mL-1，线性关系良好（ r>0.9918），回收率RSD为88.5％~123.4％，精密度RSD为1.03％~7.50％。结论本方法有良好的准确性，适用于糖尿病患者血、尿中游离氨基酸含量的测定。
목적：건립이류청산분지（ PITC）주전연생고효액상법측정당뇨병환자혈장화뇨액중유리안기산함량。방법용Waters-e2695조작계통，PITC위주전연생시제，색보주위Shim-pack VP-ODS（250 mm ×4.6 mm，5μm），류동상위갑순-을정-수화작산납용액，용작산조pH지6.5，제도세탈，용자외254 nm검측。결과해방법선성범위위2~200μg· mL-1，선성관계량호（ r>0.9918），회수솔RSD위88.5％~123.4％，정밀도RSD위1.03％~7.50％。결론본방법유량호적준학성，괄용우당뇨병환자혈、뇨중유리안기산함량적측정。
Objective To establish a pre -column derivatization and HPLC method for the determination of free amino acids in serum and urine with phenyl isothiocyanate ( PITC ) as derivatization reagent . Methods The determination was performed on waters e 2695 sys-tem.Sample was separated by Shim -pack VP-ODS column(250 mm × 4.6 mm,5 μm ) with a gradient elution of mobile phases composed of methanol/acetate/H2 O( pH 6.5 ) , the content of amino acid were tested by UV at 254 nm.Results The developed method had a good linear ( r>0.991 8 ) in the content range of 2-200 μg· mL-1 , with an aver-age recovery ranged from 88.5% to 123.4%.The precision was within 0.12%-3.42%.Conclusion PITC pre-column derivatization and HPLC method was developed and validated , which had good accuracy , suitable for the determination amino acid in serum and urine of patients with diabetes .