CAJ | 학술논문

目的：探讨甲泼尼龙琥珀酸钠鞘内注射对大鼠急性脊髓损伤后生长相关蛋白-43（GAP-43）表达的影响。方法实验分为治疗组、观察组、对照组，每组30只。治疗组予以脊髓损伤后鞘内注射3 mg · kg-1甲泼尼龙琥珀酸钠+0.9％氯化钠0.1 mL；观察组予以单纯手术暴露脊髓，不损伤脊髓；对照组予以脊髓损伤后鞘内注射0.9％氯化钠0.1 mL。用免疫组织化学方法检测脊髓损伤后各组不同时段脊髓前角细胞中表达GAP-43的细胞个数。各组大鼠在存活期间作后肢Basso Beattie Bresnahan （ BBB）运动功能评分。用苏木精-伊红染色法观察各组脊髓组织病理变化。结果免疫组织化学方法结果显示，甲泼尼龙琥珀酸钠鞘内注射增加了脊髓前角细胞GAP-43的表达。 BBB评分结果提示，甲泼尼龙琥珀酸钠鞘内注射可改善急性脊髓损伤后肢大鼠运动功能。苏木精-伊红染色结果显示，甲泼尼龙琥珀酸钠鞘内注射可减轻脊髓损伤后的病理变化。结论甲泼尼龙琥珀酸钠鞘内注射可以促进损伤脊髓前角细胞修复，其作用机制可能与甲泼尼龙琥珀酸钠鞘内注射增强损伤脊髓前角细胞GAP-43的表达有关。
목적：탐토갑발니룡호박산납초내주사대대서급성척수손상후생장상관단백-43（GAP-43）표체적영향。방법실험분위치료조、관찰조、대조조，매조30지。치료조여이척수손상후초내주사3 mg · kg-1갑발니룡호박산납+0.9％록화납0.1 mL；관찰조여이단순수술폭로척수，불손상척수；대조조여이척수손상후초내주사0.9％록화납0.1 mL。용면역조직화학방법검측척수손상후각조불동시단척수전각세포중표체GAP-43적세포개수。각조대서재존활기간작후지Basso Beattie Bresnahan （ BBB）운동공능평분。용소목정-이홍염색법관찰각조척수조직병리변화。결과면역조직화학방법결과현시，갑발니룡호박산납초내주사증가료척수전각세포GAP-43적표체。 BBB평분결과제시，갑발니룡호박산납초내주사가개선급성척수손상후지대서운동공능。소목정-이홍염색결과현시，갑발니룡호박산납초내주사가감경척수손상후적병리변화。결론갑발니룡호박산납초내주사가이촉진손상척수전각세포수복，기작용궤제가능여갑발니룡호박산납초내주사증강손상척수전각세포GAP-43적표체유관。
Objective To research the effect of intrathecal injection of methylprednisolone sodium succinate ( MPss ) on growth associated protein-43 ( GAP-43 ) expression in rats after acute spinal cord injury (SCI).Methods The rats were divided into the treatment group , the observation group and the control group , with 30 rats in each group.The rats undergone SCI in treatment group were given intrathecal injection of 3 mg· kg -1 MPss dissolved in 0.5 mL sodium chloride.The observation group received simple operation to expose the spinal cord but without damaged.The rats undergone SCI in control group were given intrathecal injection of 0.9% sodium chloride.Immunohistochemical method was used to detect the number of expressed GAP -43 in anterior spinal cord anterior horn cells at different period .The rats were tested hind limbs motion function by Basso Beattie Bresnahan ( BBB ) scores evaluation during survival time.Hematoxylin -eosin ( HE ) staining method was used to detect pathological change of injured spinal cord in each group . Results Intrathecal injection of MPss enhanced the expression of GAP-43 in anterior spinal cord anterior horn cells at different times .BBB scores proved that the function of SCI rat hind limbs movements after intrathecal injection of MPss was better .HE showed that intrathecal injection of MPss could relieve histopathological changes after spinal cord injury .Conclusion The results of immunohistochemistry show that intrathecal injection of MPss could repair anterior spinal cord anterior horn cells after spinal cord injury , the mechanism maybe that intrathecal injection of MPss could promote the expression of GAP-43 in neurons.