CAJ | 학술논문

目的：探讨血红素加氧酶-1(HO-1)与一氧化碳(CO)在肺炎支原体感染患儿中的水平变化及其意义。方法收集198例3岁以下的肺炎支原体感染患儿,根据感染患儿急性期是否伴有喘息,分为喘息组(58例)和无喘息组(140例)；喘息组中根据有无低氧血症,分为重症喘息组(24例)及轻症喘息组(34例)；而无喘息组设为对照组。采用双波长分光光度法测定患儿血COHb%(碳氧血红蛋白百分比),并以COHb%高低反映血CO含量的高低；应用ELISA技术测定患儿血HO-1含量。结果重症喘息组患儿血HO-1、COHb%分别为(2734．10±707．59) ng/L、(3．88±0．83)%；轻症喘息组患儿血HO-1、COHb%分别为(1130．03±671．02) ng/L、(1．16±0．63)%；无喘息组患儿血HO-1、COHb%分别为(931．32±451．67) ng/L、(1．04±0．37)%。重症喘息组患儿血HO-1、COHb%较轻症喘息组患儿明显增高( P均<0．01),而轻症喘息组与无喘息组患儿血HO-1、COHb%比较无明显差异( P均>0．05)。血HO-1与COHb%之间呈显著正相关(r=0．875,P<0．01)。结论肺炎支原体感染患儿血HO-1含量及COHb%随着喘息加重而增加,提示HO-1与CO可能参与了肺炎支原体感染患儿的喘息过程。
목적：탐토혈홍소가양매-1(HO-1)여일양화탄(CO)재폐염지원체감염환인중적수평변화급기의의。방법수집198례3세이하적폐염지원체감염환인,근거감염환인급성기시부반유천식,분위천식조(58례)화무천식조(140례)；천식조중근거유무저양혈증,분위중증천식조(24례)급경증천식조(34례)；이무천식조설위대조조。채용쌍파장분광광도법측정환인혈COHb%(탄양혈홍단백백분비),병이COHb%고저반영혈CO함량적고저；응용ELISA기술측정환인혈HO-1함량。결과중증천식조환인혈HO-1、COHb%분별위(2734．10±707．59) ng/L、(3．88±0．83)%；경증천식조환인혈HO-1、COHb%분별위(1130．03±671．02) ng/L、(1．16±0．63)%；무천식조환인혈HO-1、COHb%분별위(931．32±451．67) ng/L、(1．04±0．37)%。중증천식조환인혈HO-1、COHb%교경증천식조환인명현증고( P균<0．01),이경증천식조여무천식조환인혈HO-1、COHb%비교무명현차이( P균>0．05)。혈HO-1여COHb%지간정현저정상관(r=0．875,P<0．01)。결론폐염지원체감염환인혈HO-1함량급COHb%수착천식가중이증가,제시HO-1여CO가능삼여료폐염지원체감염환인적천식과정。
Objective To investigate the role of heme oxygenase-1 ( HO-1 ) and carbon monoxide ( CO) in children with mycoplasma pneumoniae infection. Methods A total of 198 cases with mycoplasma pneumoniae infection under 3 years were enrolled in this study. According to whether with wheezing,all cases were divided into wheezing group( n=58 ) and no wheezing group ( n=140 ) . According to having hypox-emia,wheezing group was divided into mild wheezing group(n=34) and severe wheezing group(n=24). And no wheezing group was set as control group. The serum COHb levels were measured by dual-wavelength spectrophotometry. The serum HO-1 were measured by ELISA. Results The levels of serum HO-1 and COHb% in severe wheezing group were ( 2 734. 10 ± 707. 59 ) ng/L, ( 3. 88 ± 0. 83 )%, and ( 1 130. 03 ± 671. 02) ng/L,(1. 16 ± 0. 63)% in mild wheezing group,and(931. 32 ± 451. 67) ng/L,(1. 04 ± 0. 37)% in no wheezing group,respectively. There were significant differences in serum HO-1 and COHb% between se-vere wheezing group and mild wheezing group(P<0. 01,respectively),and there were no significan differ-ences in serum HO-1 and COHb% between mild wheezing group and no wheezing group( P>0. 05,respec-tively). There was significant positive correlation between serum HO-1 and COHb%(r=0. 875,P<0. 01). Conclusion With mycoplasma pneumoniae infection,the expression of HO-1 and COHb% increase by the wheezing disease progression. HO-1 and CO may participate in the development process of infantile wheezing diseases.