CAJ | 학술논문

目的：研究疏肝健脾法对溃疡性结肠炎结肠黏膜中PPAR－γ基因和蛋白表达的影响。方法将60只正常大鼠随机分为正常组、模型组、研究1组、研究2组、研究3组、研究4组，每组10只。正常组不做任何处理，其他组均使用TNBS／乙醇灌肠、再用束缚法＋饮食失节的复合方法建立大鼠肝郁脾虚型溃疡性结肠炎模型。造模成功后，模型组给予0．9％氯化钠溶液灌肠，研究1组、研究2组、研究3组、研究4组分别采用5倍（10 g／kg）、10倍（20 g／kg）、15倍（30 g／kg）、20倍（40 g／kg）成人剂量的疏肝健脾的中药痛泻要方溶液灌胃治疗。治疗4周后，将大鼠集体处死，观察各组结肠黏膜情况、PPAR－γ蛋白表达平均光密度值和结肠黏膜PPAR－γ基因表达情况。结果模型组和研究1组、研究2组、研究3组、研究4组大鼠结肠黏膜PPAR－γ基因和蛋白的表达量均明显低于正常组（ P均＜0．05）；研究1组、研究2组、研究3组、研究4组结肠黏膜PPAR－γ基因和蛋白表达量均明显高于模型组（P＜0．05），研究4组结肠黏膜PPAR－γ基因和蛋白表达量均明显高于研究1组、研究2组、研究3组（ P均＜0．05）。结论溃疡性结肠炎会影响结肠黏膜中PPAR－γ基因和蛋白表达，疏肝健脾的痛泻要方中药可以提高结肠黏膜中PPAR－γ基因和蛋白表达，且呈剂量依赖性。
목적：연구소간건비법대궤양성결장염결장점막중PPAR－γ기인화단백표체적영향。방법장60지정상대서수궤분위정상조、모형조、연구1조、연구2조、연구3조、연구4조，매조10지。정상조불주임하처리，기타조균사용TNBS／을순관장、재용속박법＋음식실절적복합방법건립대서간욱비허형궤양성결장염모형。조모성공후，모형조급여0．9％록화납용액관장，연구1조、연구2조、연구3조、연구4조분별채용5배（10 g／kg）、10배（20 g／kg）、15배（30 g／kg）、20배（40 g／kg）성인제량적소간건비적중약통사요방용액관위치료。치료4주후，장대서집체처사，관찰각조결장점막정황、PPAR－γ단백표체평균광밀도치화결장점막PPAR－γ기인표체정황。결과모형조화연구1조、연구2조、연구3조、연구4조대서결장점막PPAR－γ기인화단백적표체량균명현저우정상조（ P균＜0．05）；연구1조、연구2조、연구3조、연구4조결장점막PPAR－γ기인화단백표체량균명현고우모형조（P＜0．05），연구4조결장점막PPAR－γ기인화단백표체량균명현고우연구1조、연구2조、연구3조（ P균＜0．05）。결론궤양성결장염회영향결장점막중PPAR－γ기인화단백표체，소간건비적통사요방중약가이제고결장점막중PPAR－γ기인화단백표체，차정제량의뢰성。
Objective It is to study the effect of soothing liver and invigorating spleen method on PPAR-gamma correla-ted factor gene and protein in colonic mucosa of ulcerative colitis.Methods 60 normal rats were randomly divided into normal group,model group, study group I, study group II, study group III, study group IV, each group had 10 rats.All the rats ex-cept in normal group were used to establish liver stagnation and spleen deficiency type of ulcerative colitis model by the meth-ods of TNBS/ethanol enema, binding method +inproper diet.After success establish of the models, model group was given 0.9%sodium chloride solution by enema, and the study group I,study group II, study group III, study group IV were given 5 times, 10 times, 15 times, 20 times Tongxieyaofang which could sooth liver and invigorate spleen by intragastric administra-tion.After treating for 4 weeks, all the rats were killed to observe colonic mucosa injury and the average optical density value of the expression of PPAR-protein and PPAR-gene in colonic mucosa.Results The expression of PPAR-gamma correla-ted factor gene and protein in colonic mucosa of the rats in model group and the study groups were significantly lower than that in normal group (P<0.05), and the expression in the study groups were higher than that in model group (P<0.05), the expression in study group IV was higher than that in the other three study groups ( P<0.05) .Conclusion Ulcerative colitis can influence the expression of PPAR-gamma correlated factor gene and protein, soothing liver and invigorating spleen decoc-tion Tongxieyaofang can increase the expresion of PPAR-gamma correlated factor gene and protein in colonic mucosa with a dose dependence.