中国实验诊断学
中國實驗診斷學
중국실험진단학
Chinese Journal of Laboratory Diagnosis
2015年
9期
1457-1460
,共4页
胃癌%自噬%NVP-BEZ235%凋亡
胃癌%自噬%NVP-BEZ235%凋亡
위암%자서%NVP-BEZ235%조망
gastric cancer%autophagy%NVP-BEZ235%apoptosis
目的：为了研究3-MA 是否可能增强胃癌细胞对 PI3K／mTOR 抑制剂 NVP-BEZ235的敏感性。方法以胃癌 SNU16细胞系为研究对象，SNU16细胞用不同浓度的 NVP-BEZ235（10 nM、20 nM 和50 nM）处理24 h。实验分为四组：Control 组，20 nM NVP-BEZ235组，5 mM 3-MA 组，20 nM NVP-BEZ235联合5mM 3-MA 组。MTT 法用来检测不同组内细胞的生存率，Western blotting 用来检测 Cleaved Caspase-3和 Cytochrome C 的表达水平。结果MTT 结果表明 NVP-BEZ235能抑制 SNU16细胞的增殖，3-MA 增强了 NVP-BEZ235对 SNU16细胞的增殖抑制作用。Western blotting 结果表明 NVP-BEZ235增加了 Cleaved Caspase-3和 Cytochrome C 的表达，而 NVP-BEZ235联合3-MA 会进一步诱导 Cleaved Caspase-3和 Cytochrome C 蛋白的表达。结论3-MA 通过抑制自噬增加了 SNU16细胞对 NVP-BEZ235的敏感性。
目的：為瞭研究3-MA 是否可能增彊胃癌細胞對 PI3K／mTOR 抑製劑 NVP-BEZ235的敏感性。方法以胃癌 SNU16細胞繫為研究對象，SNU16細胞用不同濃度的 NVP-BEZ235（10 nM、20 nM 和50 nM）處理24 h。實驗分為四組：Control 組，20 nM NVP-BEZ235組，5 mM 3-MA 組，20 nM NVP-BEZ235聯閤5mM 3-MA 組。MTT 法用來檢測不同組內細胞的生存率，Western blotting 用來檢測 Cleaved Caspase-3和 Cytochrome C 的錶達水平。結果MTT 結果錶明 NVP-BEZ235能抑製 SNU16細胞的增殖，3-MA 增彊瞭 NVP-BEZ235對 SNU16細胞的增殖抑製作用。Western blotting 結果錶明 NVP-BEZ235增加瞭 Cleaved Caspase-3和 Cytochrome C 的錶達，而 NVP-BEZ235聯閤3-MA 會進一步誘導 Cleaved Caspase-3和 Cytochrome C 蛋白的錶達。結論3-MA 通過抑製自噬增加瞭 SNU16細胞對 NVP-BEZ235的敏感性。
목적：위료연구3-MA 시부가능증강위암세포대 PI3K／mTOR 억제제 NVP-BEZ235적민감성。방법이위암 SNU16세포계위연구대상，SNU16세포용불동농도적 NVP-BEZ235（10 nM、20 nM 화50 nM）처리24 h。실험분위사조：Control 조，20 nM NVP-BEZ235조，5 mM 3-MA 조，20 nM NVP-BEZ235연합5mM 3-MA 조。MTT 법용래검측불동조내세포적생존솔，Western blotting 용래검측 Cleaved Caspase-3화 Cytochrome C 적표체수평。결과MTT 결과표명 NVP-BEZ235능억제 SNU16세포적증식，3-MA 증강료 NVP-BEZ235대 SNU16세포적증식억제작용。Western blotting 결과표명 NVP-BEZ235증가료 Cleaved Caspase-3화 Cytochrome C 적표체，이 NVP-BEZ235연합3-MA 회진일보유도 Cleaved Caspase-3화 Cytochrome C 단백적표체。결론3-MA 통과억제자서증가료 SNU16세포대 NVP-BEZ235적민감성。
Objective To study whether 3-MA can enhance the sensitivity of inhibitor NVP-BEZ235 to gastric canc-er cell.Methods SNU16 cells were treated by different coneentrations of NVP-BEZ235(10 nM,20 nM and 50 nM)for 24 h.Next,SNU16 cells were divided into four groups:Control group,20 nM NVP-BEZ235 group,5 mM 3-MA group, 20 nM NVP-BEZ235 combined with 5 mM 3-MA group.MTT assay was used to examine the proliferation inhibition of SNU16 cells.Western blotting was used to detect a change in protein level of Cleaved Caspase-3 and Cytochrome C.Re-sults MTT assay showed that growth inhibition rates among the groups with different levels of NVP-BEZ235 was sta-tistically significant.NVP-BEZ235 combined with 3-MA enhanced the inhibition ratio of cell compared with NVP-BEZ235 group.Western blotting showed that Cleaved Caspase-3 and Cytochrome C were increased in cells following treated with NVP-BEZ235,and then 3-MA enhanced the expression of these proteins induced by NVP-BEZ235.Conclu-sion 3-MA sensitized SNU16 cells to NVP-BEZ235 by inhibition of auto-phagy.
