CAJ | 학술논문

目的：探讨异丙酚复合氯胺酮麻醉引起幼年大鼠海马神经元以及认知功能损害后海马组织蛋白组学的变化，了解异丙酚脑保护作用的机制。方法将出生后7 d幼鼠80只随机分为生理盐水组、氯胺酮组、氯胺酮＋异丙酚低剂量组、氯胺酮＋异丙酚高剂量组，除生理盐水组外，其余组均每隔2 h给予相应药物腹腔注射1次，连续给药3次后，每组处死10只大鼠进行大脑海马组织切片及海马蛋白提取，应用MALDI－TOF指纹图谱检测系统进行蛋白组学差异研究。各组剩余的10只大鼠进行水迷宫实验，之后处死进行海马蛋白检测。结果在3d时，氯胺酮组潜伏期显著高于生理盐水组，穿环次数显著低于生理盐水组；氯胺酮＋异丙酚低剂量组和氯胺酮＋异丙酚高剂量组潜伏期明显低于氯胺酮组，氯胺酮＋异丙酚高剂量组明显低于氯胺酮＋异丙酚低剂量组；氯胺酮＋异丙酚低剂量组和氯胺酮＋异丙酚高剂量组穿环次数显著高于氯胺酮组，氯胺酮＋异丙酚高剂量组与生理盐水组和氯胺酮＋异丙酚低剂量组比较差异均无统计学意义。氯胺酮组海马神经元凋亡指数明显高于生理盐水组，氯胺酮＋异丙酚低剂量组和氯胺酮＋异丙酚高剂量组均明显低于氯胺酮组，但氯胺酮＋异丙酚低剂量组仍明显高于生理盐水组，氯胺酮＋异丙酚高剂量组与生理盐水组比较差异无统计学意义。氯胺酮组海马组织蛋白浓度明显低于生理盐水组，氯胺酮＋异丙酚低剂量组和高剂量组与氯胺酮组相比差异均无统计学意义。氯胺酮＋异丙酚组在末次给药后检测海马组织蛋白，发现上调蛋白并鉴定为PD1A3、NDUFB10、HSPA8、ATP5JD、PSMA1、isoform－CRA －c，下调蛋白为PPIA、PKM2、GFAP、NSE、SYN1；21d后检测结果表达上调蛋白为FUBP3、PRDX5，下调蛋白为GAPDH、AKR1A1、VCP、TUBULIN A1 B。结论本实验中蛋白质组学技术初步筛选了异丙酚及氯胺酮麻醉后海马组织蛋白的差异表达，为预防和改善海马神经元变化后认知功能损害提供了理论基础。目的：探討異丙酚複閤氯胺酮痳醉引起幼年大鼠海馬神經元以及認知功能損害後海馬組織蛋白組學的變化，瞭解異丙酚腦保護作用的機製。方法將齣生後7 d幼鼠80隻隨機分為生理鹽水組、氯胺酮組、氯胺酮＋異丙酚低劑量組、氯胺酮＋異丙酚高劑量組，除生理鹽水組外，其餘組均每隔2 h給予相應藥物腹腔註射1次，連續給藥3次後，每組處死10隻大鼠進行大腦海馬組織切片及海馬蛋白提取，應用MALDI－TOF指紋圖譜檢測繫統進行蛋白組學差異研究。各組剩餘的10隻大鼠進行水迷宮實驗，之後處死進行海馬蛋白檢測。結果在3d時，氯胺酮組潛伏期顯著高于生理鹽水組，穿環次數顯著低于生理鹽水組；氯胺酮＋異丙酚低劑量組和氯胺酮＋異丙酚高劑量組潛伏期明顯低于氯胺酮組，氯胺酮＋異丙酚高劑量組明顯低于氯胺酮＋異丙酚低劑量組；氯胺酮＋異丙酚低劑量組和氯胺酮＋異丙酚高劑量組穿環次數顯著高于氯胺酮組，氯胺酮＋異丙酚高劑量組與生理鹽水組和氯胺酮＋異丙酚低劑量組比較差異均無統計學意義。氯胺酮組海馬神經元凋亡指數明顯高于生理鹽水組，氯胺酮＋異丙酚低劑量組和氯胺酮＋異丙酚高劑量組均明顯低于氯胺酮組，但氯胺酮＋異丙酚低劑量組仍明顯高于生理鹽水組，氯胺酮＋異丙酚高劑量組與生理鹽水組比較差異無統計學意義。氯胺酮組海馬組織蛋白濃度明顯低于生理鹽水組，氯胺酮＋異丙酚低劑量組和高劑量組與氯胺酮組相比差異均無統計學意義。氯胺酮＋異丙酚組在末次給藥後檢測海馬組織蛋白，髮現上調蛋白併鑒定為PD1A3、NDUFB10、HSPA8、ATP5JD、PSMA1、isoform－CRA －c，下調蛋白為PPIA、PKM2、GFAP、NSE、SYN1；21d後檢測結果錶達上調蛋白為FUBP3、PRDX5，下調蛋白為GAPDH、AKR1A1、VCP、TUBULIN A1 B。結論本實驗中蛋白質組學技術初步篩選瞭異丙酚及氯胺酮痳醉後海馬組織蛋白的差異錶達，為預防和改善海馬神經元變化後認知功能損害提供瞭理論基礎。
목적：탐토이병분복합록알동마취인기유년대서해마신경원이급인지공능손해후해마조직단백조학적변화，료해이병분뇌보호작용적궤제。방법장출생후7 d유서80지수궤분위생리염수조、록알동조、록알동＋이병분저제량조、록알동＋이병분고제량조，제생리염수조외，기여조균매격2 h급여상응약물복강주사1차，련속급약3차후，매조처사10지대서진행대뇌해마조직절편급해마단백제취，응용MALDI－TOF지문도보검측계통진행단백조학차이연구。각조잉여적10지대서진행수미궁실험，지후처사진행해마단백검측。결과재3d시，록알동조잠복기현저고우생리염수조，천배차수현저저우생리염수조；록알동＋이병분저제량조화록알동＋이병분고제량조잠복기명현저우록알동조，록알동＋이병분고제량조명현저우록알동＋이병분저제량조；록알동＋이병분저제량조화록알동＋이병분고제량조천배차수현저고우록알동조，록알동＋이병분고제량조여생리염수조화록알동＋이병분저제량조비교차이균무통계학의의。록알동조해마신경원조망지수명현고우생리염수조，록알동＋이병분저제량조화록알동＋이병분고제량조균명현저우록알동조，단록알동＋이병분저제량조잉명현고우생리염수조，록알동＋이병분고제량조여생리염수조비교차이무통계학의의。록알동조해마조직단백농도명현저우생리염수조，록알동＋이병분저제량조화고제량조여록알동조상비차이균무통계학의의。록알동＋이병분조재말차급약후검측해마조직단백，발현상조단백병감정위PD1A3、NDUFB10、HSPA8、ATP5JD、PSMA1、isoform－CRA －c，하조단백위PPIA、PKM2、GFAP、NSE、SYN1；21d후검측결과표체상조단백위FUBP3、PRDX5，하조단백위GAPDH、AKR1A1、VCP、TUBULIN A1 B。결론본실험중단백질조학기술초보사선료이병분급록알동마취후해마조직단백적차이표체，위예방화개선해마신경원변화후인지공능손해제공료이론기출。
Objective It is to investigate the cause of propofol combined with ketamine anesthesia juvenile rat hippocampal neurons and cognitive dysfunction in hippocampal tissue proteomic changes, revealing changes in the pathogenesis of hipp-ocampal neurons,providing guidance for improving clinical anesthesia safety .Mtehods 80 rats after birth 7d were divided into saline group ( N group) , ketamine group ( K group) , propofol+ketamine group ( P+K group) low and high dose group chil-dren anesthesia simulation and carried hippocampus and hippocampal slices protein extraction , two-dimensional electropho-resis applications and matrix assisted laser desorption/ionization-time of flight mass spectrometry ( MALDI-TOF) proteomic differences between studies.Results The water maze reaction spell cognitive function; N hippocampal neuronal apoptosis in-dex, K group, compared with the N group (P<0.01), significant statistical significance, while the P +K group and K group was significantly lower compared to the significantly reduced apoptosis (P<0.05), but the low -dose group P +K is still high compared with the N group, the difference was significant (P<0.05), and P +K high dose groups was not signifi-cant.K hippocampal tissue protein concentration , and the NS group,compared with a significant difference.P +K and K group compared with the group with an increasing trend compared with the N -group difference was not significant.K +P group to detect protein in the hippocampus 6h, raised protein found and identified as PD1A3, NDUFB10, HSPA8, ATP5JD, PSMA1, reduced protein PPIA, PKM2, GFAP, NSE, PPIA, PKM2, GFAP and other test results after expressing 21d regula-ted protein FUBP3, PRDX5, down protein GAPDH, AKR1A1, VCP.Conclusion In this study, a preliminary screening tech-nique in proteomics propofol and ketamine anesthesia differentially expressed in hippocampal tissue protein, in order to im-prove the prevention and changes in cognitive function in hippocampal neurons provide a theoretical basis for damages .