CAJ | 학술논문

目的：探讨 RhoGDI2与 EMT 相关蛋白（E-cadherin、Vimentin）在结直肠癌组织中表达的临床意义以及它们之间的相关性。方法收集行手术切除且随访满5年的结直肠癌患者组织标本80例。应用免疫组化法检测标本中 RhoGDI2与 EMT 相关蛋白的表达情况，分析它们与各临床病理参数间的关系，探讨 RhoGDI2与 EMT 相关蛋白间的相关性。结果 RhoGDI2、E-cadherin、Vimentin 在结直肠癌组织中的阳性表达率分别为27．5％、23．6％、48.7％。结直肠癌组织中，RhoGDI2的表达与肿瘤的浸润深度、淋巴结转移、远处转移、TNM 分期及5年生存率相关（P ＜0．05）；E-cadherin 的表达与肿瘤大小、分化程度、浸润深度、淋巴结转移、TNM 分期及5年生存率相关（P ＜0.05）；Vimentin 的表达与肿瘤大小、分化程度、淋巴结转移、远处转移、TNM 分期及5年生存率相关（P ＜0．05）。结直肠癌组织中，RhoGDI2与 Vimentin 的表达呈正相关（r＝0．295，P ＜0．05）；RhoGDI2、Vimentin 与 E-cadherin 的表达呈负相关（r＝－0．278、r＝－0．309，P ＜0．05）。结论RhoGDI2与 EMT 相关蛋白在结直肠癌组织中的表达密切相关，我们推测 RhoGDI2可能通过促进 EMT 的发生导致了结直肠癌的侵袭、转移及不良预后。
목적：탐토 RhoGDI2여 EMT 상관단백（E-cadherin、Vimentin）재결직장암조직중표체적림상의의이급타문지간적상관성。방법수집행수술절제차수방만5년적결직장암환자조직표본80례。응용면역조화법검측표본중 RhoGDI2여 EMT 상관단백적표체정황，분석타문여각림상병리삼수간적관계，탐토 RhoGDI2여 EMT 상관단백간적상관성。결과 RhoGDI2、E-cadherin、Vimentin 재결직장암조직중적양성표체솔분별위27．5％、23．6％、48.7％。결직장암조직중，RhoGDI2적표체여종류적침윤심도、림파결전이、원처전이、TNM 분기급5년생존솔상관（P ＜0．05）；E-cadherin 적표체여종류대소、분화정도、침윤심도、림파결전이、TNM 분기급5년생존솔상관（P ＜0.05）；Vimentin 적표체여종류대소、분화정도、림파결전이、원처전이、TNM 분기급5년생존솔상관（P ＜0．05）。결직장암조직중，RhoGDI2여 Vimentin 적표체정정상관（r＝0．295，P ＜0．05）；RhoGDI2、Vimentin 여 E-cadherin 적표체정부상관（r＝－0．278、r＝－0．309，P ＜0．05）。결론RhoGDI2여 EMT 상관단백재결직장암조직중적표체밀절상관，아문추측 RhoGDI2가능통과촉진 EMT 적발생도치료결직장암적침습、전이급불량예후。
Objective To investigate the clinical significance and correlation of RhoGDI2 and EMT-related proteins (E-cadherin,Vimentin)expression in colorectal carcinoma tissues.Methods The tissue specimens from 80 patients that were surgical resected for CRC and follow-up for over 5 years.The expression of RhoGDI2 and EMT-related proteins (E-cadherin,Vimentin)were assessed by immunohistochemistry.The associations of their expression with clinicopatho-logical parameters and themselves were analyzed.Results The positive expression rate of RhoGDI2,E-cadherin and Vi-mentin was 27.5%,23.6%,48.7% respectively in CRC tissues.In CRC tissues,the expression of RhoGDI2 were asso-ciated with the depth of invasion,lymph node metastasis,distant metastasis,TNM stage and 5-year survival(P <0.05);the expression of E-cadherin were associated with the tumor size,differentiation,depth of invasion,lymph node metasta-sis,TNM stage and 5-year survival(P <0.05);the expression of Vimentin were associated with the tumor size,differen-tiation,lymph node metastasis,distant metastasis,TNM stage and 5-year survival(P <0.05).The expression of RhoG-DI2 with Vimentin showed positive correlation(r=0.295,P <0.05);the expression of RhoGDI2,Vimentin with E-cad-herin showed negative correlation(r=-0.278,r=-0.309,P <0.05).Conclusion The expression of RhoGDI2 was significantly associated with EMT-related proteins(E-cadherin,Vimentin)in CRC tissues.We speculate that RhoGDI2 may cause CRC invasion,metastasis and poor prognosis by promoting EMT occurrence.