CAJ | 학술논문

目的：研究ACT HR高表达对人肾上腺皮质癌H295R细胞的影响，并探讨ACT HR在肾上腺肿瘤发病中的作用。方法用慢病毒包装的ACT HR高表达载体感染 H295R细胞作为实验组，同时设立对照组。用WST‐1法及细胞计数法检测感染后细胞的增殖情况，感染48 h后 TUNEL法检测细胞的凋亡情况。结果 WST‐1增殖检测发现实验组细胞增殖活性呈双重变化趋势，在前3天内增殖活性高于对照组细胞，尤其是第2天达到顶峰，但第4天后增殖活性低于对照组细胞；细胞计数法显示在增殖活性最高时，实验组和对照组细胞分别为（5．3±0．21）×103和（3．8±0．24）×103个，两组细胞计数结果对比具有统计学意义（P＜0．05）；而TUNEL结果显示荧光镜下两组细胞的凋亡情况无明显差异（P＞0．05）。结论 ACTHR对肾上腺肿瘤的影响具有时间依赖性，但可能主要以促进细胞增殖为主，而对凋亡影响不明显。
목적：연구ACT HR고표체대인신상선피질암H295R세포적영향，병탐토ACT HR재신상선종류발병중적작용。방법용만병독포장적ACT HR고표체재체감염 H295R세포작위실험조，동시설립대조조。용WST‐1법급세포계수법검측감염후세포적증식정황，감염48 h후 TUNEL법검측세포적조망정황。결과 WST‐1증식검측발현실험조세포증식활성정쌍중변화추세，재전3천내증식활성고우대조조세포，우기시제2천체도정봉，단제4천후증식활성저우대조조세포；세포계수법현시재증식활성최고시，실험조화대조조세포분별위（5．3±0．21）×103화（3．8±0．24）×103개，량조세포계수결과대비구유통계학의의（P＜0．05）；이TUNEL결과현시형광경하량조세포적조망정황무명현차이（P＞0．05）。결론 ACTHR대신상선종류적영향구유시간의뢰성，단가능주요이촉진세포증식위주，이대조망영향불명현。
Objective To explore the effect of adrenocorti cotrophic hormone receptor (ACTHR) over‐expression on the proliferation of human adrenocortical H295R cells ,and to investigate its role in the pathogenesis of adrenal tumors .Methods H295R cells transfected by lentivirus‐mediated ACTHR served as the experimental group .H295R cells without virus infection served as control group .The cell proliferation at different ACTHR levels was evaluated with WST‐1 and cell count .Cell apoptosis after 48 hours was measured with TUNEL .Results Higher proliferation activity was observed in the experimental cells than in the control cells in the first three days ,which peaked on the second day .But this trend reversed on the fourth day . The number of experimental and control cells were (5 .3 ± 0 .21) × 103 and (3 .8 ± 0 .24) × 103 at peak stage ,( P < 0 .05) .By contrast ,there was no significant difference between the two groups in cell apoptosis .Conclusion ACTHR affects H295R cells in a time‐dependent manner .It has stronger effect on the proliferation than on the apoptosis of H295R cells .