CAJ | 학술논문

目的建立咳喘宁合剂中氨茶碱和吗啡含量测定的HPLC方法. 方法采用高效液相色谱法,色谱柱为资生堂C8 色谱柱,流动相为0.1 mol/L 磷酸二氢钾溶液 -0.005 mol/L 庚烷磺酸钠水溶液 -乙腈(47. 5:47. 5:5). 氨茶碱检测波长为271 nm,进样量为10 μL;吗啡检测波长为207 nm,进样量为20 μL. 结果茶碱在8. 62~68. 96 μg/mL范围内具有良好的线性关系,平均回收率为99. 2%;吗啡在1. 03 ~51. 60 μg/mL范围内具有良好的线性关系,平均回收率为101. 5%. 结论该方法具有良好的准确度、精密度、专属性,能良好地控制咳喘宁合剂的质量.
목적 건립해천저합제중안다감화마배함량측정적HPLC방법. 방법 채용고효액상색보법,색보주위자생당C8 색보주,류동상위0.1 mol/L 린산이경갑용액 -0.005 mol/L 경완광산납수용액 -을정(47. 5:47. 5:5). 안다감검측파장위271 nm,진양량위10 μL;마배검측파장위207 nm,진양량위20 μL. 결과다감재8. 62~68. 96 μg/mL범위내구유량호적선성관계,평균회수솔위99. 2%;마배재1. 03 ~51. 60 μg/mL범위내구유량호적선성관계,평균회수솔위101. 5%. 결론 해방법구유량호적준학도、정밀도、전속성,능량호지공제해천저합제적질량.
Objective To establish a method for the content determination of aminophylline and morphine in kechuanning mixture. Methods The determination was performed on Shisheido C8 column with the mobile phases of 0.1 mol/L potassium dihydrogen phosphate solution-0.005 mol/L sodium heptanesulfonate solution-acetonitrile (47. 5:47. 5:5). The detection wavelength for aminophylline was 271 nm and the sample volume was 10 μL;the detec-tion wavelength for morphine was 207 nm and the sample volume was 20 μL. Results The linear range of the theo-phylline was 8. 62~68. 96 μg/mL with an average recovery of 99. 2%;The linear range of the morphine was 1. 03~51. 60 μg/mL with an average recovery of 101. 5%. Conclusion The method is accurate,precise and specific for the quality control of aminophylline and morphine in kechuanning mixture.