实用药物与临床
實用藥物與臨床
실용약물여림상
Practical Pharmacy and Clinical Remedies
2015年
9期
1087-1089
,共3页
卡巴匹林钙%高效液相色谱法%含量测定%方法比较
卡巴匹林鈣%高效液相色譜法%含量測定%方法比較
잡파필림개%고효액상색보법%함량측정%방법비교
Carbasalate calcium%HPLC%Determination method%Method comparation
目的 建立高效液相色谱法( HPLC)测定卡巴匹林钙的含量,并和容量滴定法进行对比研究.方法 色谱柱为美国热电C18柱(250 mm × 4. 6 mm,5 μm),流动相为磷酸-乙腈-水(2:300:600)溶液,进样量20 μL,检测波长为228 nm;对两种测定方法分别进行了方法学验证研究. 结果 两种含量测定方法在考察浓度范围内均具有良好线性关系. 结论 采用高效液相色谱法测定卡巴匹林钙的含量,该法操作简便,专属性强,结果准确.
目的 建立高效液相色譜法( HPLC)測定卡巴匹林鈣的含量,併和容量滴定法進行對比研究.方法 色譜柱為美國熱電C18柱(250 mm × 4. 6 mm,5 μm),流動相為燐痠-乙腈-水(2:300:600)溶液,進樣量20 μL,檢測波長為228 nm;對兩種測定方法分彆進行瞭方法學驗證研究. 結果 兩種含量測定方法在攷察濃度範圍內均具有良好線性關繫. 結論 採用高效液相色譜法測定卡巴匹林鈣的含量,該法操作簡便,專屬性彊,結果準確.
목적 건립고효액상색보법( HPLC)측정잡파필림개적함량,병화용량적정법진행대비연구.방법 색보주위미국열전C18주(250 mm × 4. 6 mm,5 μm),류동상위린산-을정-수(2:300:600)용액,진양량20 μL,검측파장위228 nm;대량충측정방법분별진행료방법학험증연구. 결과 량충함량측정방법재고찰농도범위내균구유량호선성관계. 결론 채용고효액상색보법측정잡파필림개적함량,해법조작간편,전속성강,결과준학.
Objective To establish an HPLC method for determination of carbasalate calcium and compare with the volumetric precipitation method. Methods The Thermo C18 column(250 mm × 4. 6 mm,5 μm)was adopted, the mobile phase was the mixture of phosphoric acid-acetonitrile-water (2:300:600),the injection volume was 20 μL, and the detection wavelength was 228 nm. The two methods were validated respectively. Results Two content deter-mination methods had good linear relationship in the concentration range. Conclusion The HPLC method is simple, and with accurate and reliable results,which can be used for the determination of carbasalate calcium.
