实用药物与临床
實用藥物與臨床
실용약물여림상
Practical Pharmacy and Clinical Remedies
2015年
9期
1081-1083
,共3页
王娟%汪选斌%李洪亮%欧阳露
王娟%汪選斌%李洪亮%歐暘露
왕연%왕선빈%리홍량%구양로
高效液相色谱法%补肾化瘀浸膏%毛蕊花糖苷
高效液相色譜法%補腎化瘀浸膏%毛蕊花糖苷
고효액상색보법%보신화어침고%모예화당감
HPLC%Bushenhuayu extracts%Acteoside
目的 建立补肾化瘀浸膏中毛蕊花糖苷的HPLC含量测定方法. 方法 采用ODS Phenomenex Gemini C18柱(250 mm × 4. 6 mm,5 μm),以乙腈-0.1%醋酸溶液(16:84)为流动相,检测波长:334 nm,流速:1. 0 mL/min,柱温:30 ℃. 结果 毛蕊花糖苷进样量在0.015 ~0.300 μg 之间线性关系良好. 回归方程 Y =16 763 X-7 948. 9,R=0.999 6;平均加样回收率为98. 24%,RSD为1. 34%(n=6). 结论 本方法操作简便,测定结果准确,重复性好,可作为补肾化瘀浸膏的质量控制方法.
目的 建立補腎化瘀浸膏中毛蕊花糖苷的HPLC含量測定方法. 方法 採用ODS Phenomenex Gemini C18柱(250 mm × 4. 6 mm,5 μm),以乙腈-0.1%醋痠溶液(16:84)為流動相,檢測波長:334 nm,流速:1. 0 mL/min,柱溫:30 ℃. 結果 毛蕊花糖苷進樣量在0.015 ~0.300 μg 之間線性關繫良好. 迴歸方程 Y =16 763 X-7 948. 9,R=0.999 6;平均加樣迴收率為98. 24%,RSD為1. 34%(n=6). 結論 本方法操作簡便,測定結果準確,重複性好,可作為補腎化瘀浸膏的質量控製方法.
목적 건립보신화어침고중모예화당감적HPLC함량측정방법. 방법 채용ODS Phenomenex Gemini C18주(250 mm × 4. 6 mm,5 μm),이을정-0.1%작산용액(16:84)위류동상,검측파장:334 nm,류속:1. 0 mL/min,주온:30 ℃. 결과 모예화당감진양량재0.015 ~0.300 μg 지간선성관계량호. 회귀방정 Y =16 763 X-7 948. 9,R=0.999 6;평균가양회수솔위98. 24%,RSD위1. 34%(n=6). 결론 본방법조작간편,측정결과준학,중복성호,가작위보신화어침고적질량공제방법.
Objective To establish a method for determination of acteoside in Bushenhuayu extracts by HPLC. Methods HPLC was conducted at a column temperature of 30 ℃ with a column of ODS Phenomenex Gemini C18(250 mm × 4. 6 mm,5 μm),the mobile phase consisted of acetonitrile-0.1% acetic acid (16:84 V/V),wave length was set at 334 nm and the flow rate was 1. 0 mL/min. Results There was a good linear ranging from 0.015~0.300 μg of acteoside. The curve formulation was Y=16 763 X-7 948. 9,R=0.999 6,with an average recovery of 98. 24% (RSD=1. 34%,n=6). Conclusion The method might be one of the good quality criterion for Bushenhuayu extract because of its simple,accurate and recovery property.
