世界复合医学
世界複閤醫學
세계복합의학
World Journal of Complex Medicine
2015年
3期
227-230
,共4页
刘海波%孙海飚%韩晓强%卢向东%韩树峰%王彤
劉海波%孫海飚%韓曉彊%盧嚮東%韓樹峰%王彤
류해파%손해표%한효강%로향동%한수봉%왕동
血清素%成骨细胞%分化
血清素%成骨細胞%分化
혈청소%성골세포%분화
serotonin%osteoblasts%differentiation
目的:研究不同浓度的血清素对体外培养大鼠成骨细胞分化的影响。方法:分离培养新生SD大鼠颅骨的成骨细胞,分别向培养液中加入不同浓度的血清素,分为4组:0 mol/L组(空白对照组)、10-9 mol/L组、10-8 mol/L组、10-7 mol/L组,通过RT-PCR检测骨钙素(OCN),碱性磷酸酶( ALP),Runt相关转录因子-2(Runx 2)和Osterix mRNA相对表达量的变化。结果:不同浓度的血清素均可以抑制骨钙素(OCN),碱性磷酸酶( ALP),Runt相关转录因子-2(Runx 2)和Osterix mRNA的相对表达量,且这种抑制作用随着血清素浓度的增大而增强。结论:血清素可以剂量依赖性地抑制成骨细胞的分化,这为骨质疏松症的治疗提供了新的思路。
目的:研究不同濃度的血清素對體外培養大鼠成骨細胞分化的影響。方法:分離培養新生SD大鼠顱骨的成骨細胞,分彆嚮培養液中加入不同濃度的血清素,分為4組:0 mol/L組(空白對照組)、10-9 mol/L組、10-8 mol/L組、10-7 mol/L組,通過RT-PCR檢測骨鈣素(OCN),堿性燐痠酶( ALP),Runt相關轉錄因子-2(Runx 2)和Osterix mRNA相對錶達量的變化。結果:不同濃度的血清素均可以抑製骨鈣素(OCN),堿性燐痠酶( ALP),Runt相關轉錄因子-2(Runx 2)和Osterix mRNA的相對錶達量,且這種抑製作用隨著血清素濃度的增大而增彊。結論:血清素可以劑量依賴性地抑製成骨細胞的分化,這為骨質疏鬆癥的治療提供瞭新的思路。
목적:연구불동농도적혈청소대체외배양대서성골세포분화적영향。방법:분리배양신생SD대서로골적성골세포,분별향배양액중가입불동농도적혈청소,분위4조:0 mol/L조(공백대조조)、10-9 mol/L조、10-8 mol/L조、10-7 mol/L조,통과RT-PCR검측골개소(OCN),감성린산매( ALP),Runt상관전록인자-2(Runx 2)화Osterix mRNA상대표체량적변화。결과:불동농도적혈청소균가이억제골개소(OCN),감성린산매( ALP),Runt상관전록인자-2(Runx 2)화Osterix mRNA적상대표체량,차저충억제작용수착혈청소농도적증대이증강。결론:혈청소가이제량의뢰성지억제성골세포적분화,저위골질소송증적치료제공료신적사로。
Objective: to study the effects of serotonin at different concentrations on differentiation and of cultured primary rat osteoblasts in vitro. Methods: the osteoblasts from the skull of newborn SD rats, were divied into four groups by the culture medium with different serotonin concentrations: 0mol/L group(blank control), 10-9mol/L group, 10-8mol/Lgroup and 10-7mol/L group. In addition, mRNA expression of osteocalcin(OCN), alkaline phosphatase(ALP), runt-related transcription factor2 (Runx2) and Osterix were quantitated by real-time reverse transcription polymerase chain reaction. Results: different concentrations of serotonin inhibited the mRNA expression of OCN, ALP, Runx2and Osterix of osteoblasts; And, the inhibitory effect of serotonin was dose-dependent. Conclusion:serotonin can inhibit differentiation of osteoblasts; which provide a novel approach for the treatments of osteoporosis.