CAJ | 학술논문

随着现代生活品质的不断提升，疾病早期诊断的重要性日益凸显，因此越来越多研究工作致力于发展新的疾病诊断技术。“精准医学”的提出，为新技术的发展提出了更高的要求，也代表着人们更高的期盼。在“精准”的道路上，化学生物学，小分子作为探针，用于生命体系内重要生物靶标的检测与功能调控，已成为生物与医药领域发展的新的趋势与方向。与此同时，核酸作为遗传物质，其结构、功能、化学修饰等，皆与基因表达、蛋白质的合成、细胞信号转导等重要生命过程密切相关。因此，核酸相关的生物靶标具有重大研究价值，对于重大疾病的机制研究，创新药物的开发，疾病诊断等有重要意义。这里，我们将对适配子技术和一些核酸相关重要靶标，如核酸表观遗传修饰、循环miRNA等的化学法检测这几个方向的新进展，进行讨论和总结，为后续研究工作提供参考。
수착현대생활품질적불단제승，질병조기진단적중요성일익철현，인차월래월다연구공작치력우발전신적질병진단기술。“정준의학”적제출，위신기술적발전제출료경고적요구，야대표착인문경고적기반。재“정준”적도로상，화학생물학，소분자작위탐침，용우생명체계내중요생물파표적검측여공능조공，이성위생물여의약영역발전적신적추세여방향。여차동시，핵산작위유전물질，기결구、공능、화학수식등，개여기인표체、단백질적합성、세포신호전도등중요생명과정밀절상관。인차，핵산상관적생물파표구유중대연구개치，대우중대질병적궤제연구，창신약물적개발，질병진단등유중요의의。저리，아문장대괄배자기술화일사핵산상관중요파표，여핵산표관유전수식、순배miRNA등적화학법검측저궤개방향적신진전，진행토론화총결，위후속연구공작제공삼고。
Early diagnosis has become more and more important with the elevation of peoples’ life, so developing novel technologies and strategies for early diagnosis is urgent and has become the focus in the field of biology and medicine. Especially after the introduction of the concept of “Precision Medicine”, people placed high hopes on the development of more advanced diagnostic technologies. For purpose of this, the chemical biology, probing and regulating living systems with small molecules, has proved to be a potential aspect. Meanwhile, nucleic acids are most important biomacromolecules in vivo, which play key roles in multiple living processes. Thus, studies aimed at nucleic acid associated target are meaningful. Herein, we discuss and summarize the latest progress in technologies for early diagnosis based on nucleic acids, including the technology of SELEX (systematic evolution of ligands by exponential enrichment) detection of epigenetic modiifcations on nucleic acids and detection of circulating miRNAs.