CAJ | 학술논문

目的：建立一种稳定的血浆变肾上腺素（ MN）和去甲变肾上腺素（ NMN）的液相色谱－串联质谱检测方法（ LC－MS／MS）。方法方法学建立。自2012年1月至12月于复旦大学附属中山医院体检中心招募126名健康人，同时在中山医院就诊的泌尿外科和内分泌科患者中入组疾病组21例（嗜铬细胞瘤17例，异位嗜铬细胞瘤4例），高血压组108例，疾病对照组84例。以同位素氘代作为内标，采用BEH HILIC色谱柱进行分离。流动相为甲酸铵缓冲液（100 mmol／L，pH3）和纯乙腈，梯度洗脱；柱温：35℃；使用Waters? XevoTM TQ MS ACQUITY UPLC? System建立方法，并对该方法的线性，回收率，精密度，最低检测下限进行性能评价。比较各组同健康人组MN和NMN水平的差异。结果该方法通过了线性，回收率，精密度和最低检测下限的性能评价。嗜铬细胞瘤组同健康人组的MN和NMN水平有显著统计学差异。结论建立的LC－MS／MS方法检测MN和NMN，适合于临床应用。（中华检验医学杂志，2015，38：605－608）
목적：건립일충은정적혈장변신상선소（ MN）화거갑변신상선소（ NMN）적액상색보－천련질보검측방법（ LC－MS／MS）。방법방법학건립。자2012년1월지12월우복단대학부속중산의원체검중심초모126명건강인，동시재중산의원취진적비뇨외과화내분비과환자중입조질병조21례（기락세포류17례，이위기락세포류4례），고혈압조108례，질병대조조84례。이동위소도대작위내표，채용BEH HILIC색보주진행분리。류동상위갑산안완충액（100 mmol／L，pH3）화순을정，제도세탈；주온：35℃；사용Waters? XevoTM TQ MS ACQUITY UPLC? System건립방법，병대해방법적선성，회수솔，정밀도，최저검측하한진행성능평개。비교각조동건강인조MN화NMN수평적차이。결과해방법통과료선성，회수솔，정밀도화최저검측하한적성능평개。기락세포류조동건강인조적MN화NMN수평유현저통계학차이。결론건립적LC－MS／MS방법검측MN화NMN，괄합우림상응용。（중화검험의학잡지，2015，38：605－608）
Objective The aim of our study was to develop a robust LC-MS/MS method for determination of MN and NMN in blood plasma.Methods A liquid chromatography -tandem mass spectrometric ( LC-MS/MS) method was used, with signal linearity, lower limits of quantitation, precision and accuracy being evaluated.The study recruited 126 healthy volunteers, and MN and NMN in blood plasma were determined.At the same time samples from 21 patients ( 17 pheochromocytoma, 4 ectopic pheochromocytoma) , a hypertension group of 108 persons, and a control group of 84 persons were analyzed. A paired T test was used to compare the MN and NMN levels between the different groups.Results The performance characteristics for the method in terms of linearity, lower limits of quantitation, precision and accuracy were verified.Significant differences were found between the concentration levels of MN and NMN in the diseased and healthy groups.Conclusion A robust and reliable LC-MS/MS method for the determination of MN and NMN in blood plasma has been developed and was shown to be suitable for clinical application.