中华检验医学杂志
中華檢驗醫學雜誌
중화검험의학잡지
Chinese Journal of Laboratory Medicine
2015年
9期
592-594
,共3页
聚合酶链反应%微流体分析技术%早期诊断%产前诊断
聚閤酶鏈反應%微流體分析技術%早期診斷%產前診斷
취합매련반응%미류체분석기술%조기진단%산전진단
Polymerase chain reaction%Microfluidic analytical techniques%Early diagnosis%Prenatal diagnosis
微流控芯片数字PCR采用微流控芯片,将PCR反应液分割成数量众多且体积相等的反应单元,通过检测扩增后荧光信号呈阳性的反应单元的数量对核酸模板进行定量,具有可绝对定量、灵敏度高等优点。在感染性疾病诊断、肿瘤早期诊断、产前诊断等多个领域显示出巨大的应用前景,已有多家公司相继推出各自产品。相信随着技术的进步和新产品的出现,其应用范围将不断扩展,有望成为新一代的基因诊断工具。(中华检验医学杂志,2015,38:592-594)
微流控芯片數字PCR採用微流控芯片,將PCR反應液分割成數量衆多且體積相等的反應單元,通過檢測擴增後熒光信號呈暘性的反應單元的數量對覈痠模闆進行定量,具有可絕對定量、靈敏度高等優點。在感染性疾病診斷、腫瘤早期診斷、產前診斷等多箇領域顯示齣巨大的應用前景,已有多傢公司相繼推齣各自產品。相信隨著技術的進步和新產品的齣現,其應用範圍將不斷擴展,有望成為新一代的基因診斷工具。(中華檢驗醫學雜誌,2015,38:592-594)
미류공심편수자PCR채용미류공심편,장PCR반응액분할성수량음다차체적상등적반응단원,통과검측확증후형광신호정양성적반응단원적수량대핵산모판진행정량,구유가절대정량、령민도고등우점。재감염성질병진단、종류조기진단、산전진단등다개영역현시출거대적응용전경,이유다가공사상계추출각자산품。상신수착기술적진보화신산품적출현,기응용범위장불단확전,유망성위신일대적기인진단공구。(중화검험의학잡지,2015,38:592-594)
Microfluidics-based digital PCR depends on microfluidic chip to split PCR reaction mixture into many tiny equal-volume units.Quantitative assessment of target DNA template can be obtained by counting the number of fluorescence-positive units after thermocycling.Microfluidics-based digital PCR exhibits many advantages including absolute quantification, high sensitivity and accuracy, and shows great promise in a variety of applications, such as infectious diseases diagnose, early cancer detection and prenatal diagnose. There are already several microfludics-based digital PCR products produced from sereval companies.It is believed that as the technology improves, microfluidics-based digital PCR will find broader applications and become the next-generation tool for genetic tests.