CAJ | 학술논문

目的：探讨赤芍总苷对非小细胞肺癌模型大鼠 MDR1-mRNA、MRP-mRNA及LRP-mRNA基因表达影响效果。方法取50只雄性大鼠随机分为正常组5只，给予0.9％氯化钠；剩下45只大鼠分别构建鳞状细胞癌、腺癌、大细胞癌肺癌模型各15只，各模型构建成功后分别分为模型组、赤芍总苷组和环磷酰胺组，每组各5只。模型组背静脉注射0.9％氯化钠1 mL；赤芍总苷组每天背静脉注射赤芍总苷240 mg · kg-1；环磷酰胺组每天背静脉注射环磷酰胺0.4 mL· kg-1，各组均连续治疗7 d。试验结束后，处死各组大鼠，并取尾静脉血及肺组织，进行肿瘤标记物、白细胞水平检测，制备肺组织切片，并通过聚合酶链反应法检测MDR1-mRNA、MRP-mRNA及LRP-mRNA基因表达水平。结果赤芍总苷组和环磷酰胺组大鼠与模型组相比，癌胚抗原、糖蛋白抗原50水平、白细胞水平和白细胞最低值均有不同程度降低，其中赤芍总苷组癌胚抗原、糖蛋白抗原50水平显著低于环磷酰胺组（ P＜0.05），而赤芍总苷组白细胞和白细胞最低值较环磷酰胺组明显增高（ P＜0.05）。赤芍总苷组MDR1基因、MRP基因和LRP基因较环磷酰胺组表达量均明显增加（ P＜0.05）。结论赤芍总苷能够明显抑制肿瘤细胞，并降低肺耐药蛋白及多药耐药基因的表达，从而提高机体对药物的耐受性和敏感性。
목적：탐토적작총감대비소세포폐암모형대서 MDR1-mRNA、MRP-mRNA급LRP-mRNA기인표체영향효과。방법취50지웅성대서수궤분위정상조5지，급여0.9％록화납；잉하45지대서분별구건린상세포암、선암、대세포암폐암모형각15지，각모형구건성공후분별분위모형조、적작총감조화배린선알조，매조각5지。모형조배정맥주사0.9％록화납1 mL；적작총감조매천배정맥주사적작총감240 mg · kg-1；배린선알조매천배정맥주사배린선알0.4 mL· kg-1，각조균련속치료7 d。시험결속후，처사각조대서，병취미정맥혈급폐조직，진행종류표기물、백세포수평검측，제비폐조직절편，병통과취합매련반응법검측MDR1-mRNA、MRP-mRNA급LRP-mRNA기인표체수평。결과적작총감조화배린선알조대서여모형조상비，암배항원、당단백항원50수평、백세포수평화백세포최저치균유불동정도강저，기중적작총감조암배항원、당단백항원50수평현저저우배린선알조（ P＜0.05），이적작총감조백세포화백세포최저치교배린선알조명현증고（ P＜0.05）。적작총감조MDR1기인、MRP기인화LRP기인교배린선알조표체량균명현증가（ P＜0.05）。결론적작총감능구명현억제종류세포，병강저폐내약단백급다약내약기인적표체，종이제고궤체대약물적내수성화민감성。
Objective To explore the effection of total paeony glycosides on the expression of multidrug resistance gene in non -small cell lung cancer model rats.Methods Fifty male selected rats were randomly di-vided into control group ( 5 rats and only inject saline ) and model group (45 rats).The model group included squamous cell carcinoma , adeno-carcinoma and large cell carcinoma cell line , and each model contained 15 rats, when the models successfully constructed , the rats were grouped into model group , total paeony glycoside group and cyclophosphamide group.The total paeony glycoside group was injected the drug everyday through dorsal vein 240 mg · kg -1 , the model group was given intrave-nous injection of saline 1 mL and cyclophosphamide group was 0.4 mL· kg-1 for 7 days.After experiments , all the rats were executed and the vein blood and lung tissue were sampled for marking tumors , blood testing , histological section and detecting the expression levels of multidrug resistance gene 1-mRNA(MRP-mRNA), lung resistance protein -mRNA(LRP-mRNA) and multidrug resistance associated protein -mRNA ( MDR1 -mRNA ) gene by RT -PCR.Results Compared with the control group, carcino-embryonic antigen(CEA), CA50 level, white blood cell(WBC), and the minimum value of WBC all decreased to some extent in the total paeony glycoside group and cyclophosphamide group of these , the value of CEA , tumor marker 50(CA50) in total paeony glycoside group was significantly lower than values in cyclophosphamide group (P<0.05).While the minimum value of WBC was the opposite (P<0.05).Drug resistant gene in total paeony gly-coside group and cyclophosphamide group were all expressed to varying degrees , but polymerase chain reaction (PCR) product density , MDR1, MRP, and LRP gene expression in the former group all increased and larger than later group (P<0.05).Conclusion Total paeony glycoside can obviously inhibit the diffusion of tumor cells , the expression of lung resistant protein and multi -drug resistant gene , thereby enhancing the resistance and sensitivity of body to drug-sithas instructional significance for clinic .