CAJ | 학술논문

目的：研究外源性硫化氢（ H2 S）对大鼠胶质瘤血管形成的诱导机制。方法成年SD大鼠40只，随机分为4组，对照组、硫氢化钠（ NaHS）组、肿瘤组、肿瘤+NaHS组，每组10只。肿瘤组、肿瘤+NaHS组，大鼠纹状体内注射C6胶质瘤细胞，建立胶质瘤模型；对照组、NaHS组，大鼠大脑不做任何处理。4组大鼠正常饲养3周后，对其进行断头取脑，用敏感硫电极法检测各组大鼠脑组织及瘤内H2 S的含量；Real time PCR法检测胱硫醚-β-合成酶（ CBS ）、3-巯基丙酮酸硫转移酶（3-MST）的mRNA转录水平；ELISA及蛋白免疫印迹法检测缺氧诱导因子-1α（ HIF -1α）、血管内皮生长因子（ VEGF ）、基质金属蛋白酶-9（MMP-9）的表达；同时计数瘤内微血管密度（CD105-MVD）。结果肿瘤组、肿瘤+NaHS组，均可见明显的肿瘤组织，与对照组相比，瘤内毛细血管数明显增多；肿瘤+NaHS组，瘤体内有更多的HIF-α、VEGF、MMP-9的表达，且MVD明显高于对照组、NaHS组、肿瘤组（均P＜0.01）。结论外源性H2 S能诱导并促进大鼠恶性胶质瘤内的血管生成，其发生机制可能与H2 S促进CBS、3-MST、HIF-α、VEGF及MMP-9的表达密切相关。
목적：연구외원성류화경（ H2 S）대대서효질류혈관형성적유도궤제。방법성년SD대서40지，수궤분위4조，대조조、류경화납（ NaHS）조、종류조、종류+NaHS조，매조10지。종류조、종류+NaHS조，대서문상체내주사C6효질류세포，건립효질류모형；대조조、NaHS조，대서대뇌불주임하처리。4조대서정상사양3주후，대기진행단두취뇌，용민감류전겁법검측각조대서뇌조직급류내H2 S적함량；Real time PCR법검측광류미-β-합성매（ CBS ）、3-구기병동산류전이매（3-MST）적mRNA전록수평；ELISA급단백면역인적법검측결양유도인자-1α（ HIF -1α）、혈관내피생장인자（ VEGF ）、기질금속단백매-9（MMP-9）적표체；동시계수류내미혈관밀도（CD105-MVD）。결과종류조、종류+NaHS조，균가견명현적종류조직，여대조조상비，류내모세혈관수명현증다；종류+NaHS조，류체내유경다적HIF-α、VEGF、MMP-9적표체，차MVD명현고우대조조、NaHS조、종류조（균P＜0.01）。결론외원성H2 S능유도병촉진대서악성효질류내적혈관생성，기발생궤제가능여H2 S촉진CBS、3-MST、HIF-α、VEGF급MMP-9적표체밀절상관。
Objective To study the mechanism induced by exogenous H2 S on rat glioma formation.Methods Fourty adult SD rats were ran-domly divided into control group , NaHS group , tumor group , tumor +NaHS group of four groups , each group 10.Tumor group , tumor+NaHS group injected rat striatum C 6 glioma cells to established glioma model . Control group, NaHS group received no treatment.Four groups of rats fed normally , three weeks after taking the brain sensitive sulfur electrode applied to detect the rat brain tumor and H 2 S content of its decapitated . The cystathionine -beta-synthase ( CBS ) , mRNA transcription level of 3-mercaptopyruvate sulfurtransferase (3MST) were determined by real time PCR assay. The hypoxia -inducible transcription factors -1α(HIF-1α), vascular endothelial growth factor (VEGF) and the expre-ssion of matrix metallo proteinase-9 ( MMP-9 ) by ELISA and Western blot.The intratumoral micro vessel density ( MVD ) was counted by CD105 -MVD. Results Compared with the control group , NaHS group, tumor group, tumor +NaHS group were clearly visible tumor tissue, significantly increased the number of tumor capillaries .Tumor+NaHS group has more CBS , 3MST, HIF-α, VEGF expression of MMP -9.And tumor +NaHS group was significantly higher than the NaHS group , tumor group ( P<0.01 ) .Conclusion Exogenous H 2 S may induce and promote angiogenesis in rat glioma within its mechanism of H2S promote CBS, 3MST, closely related to the HIF-αVEGF expression of matrix metalloproteinase MMP -9.