CAJ | 학술논문

选用天然多糖中唯一的碱性多糖———壳聚糖作为稳定剂和包裹剂,成功合成了水溶性的AgInS2量子点/低分子量壳聚糖纳米复合材料( AgInS2/LCSMS)。利用透射电子显微镜( TEM)、FT-IR傅里叶红外光谱仪、紫外吸收光谱、荧光分光光度计等表征手段对纳米复合材料的形貌、化学组成及光学性质进行了研究。结果表明,AgInS2/LCSMS纳米复合材料的粒径约为5~6 nm,在水相中仍具有较稳定的发光。之后,对AgInS2/LCSMS纳米复合材料的生物相容性进行了研究,对比AgInS2/LCSMS纳米复合材料与AgInS2量子点的细胞活性测试结果发现,AgInS2/LCSMS纳米复合材料的细胞活性比AgInS2量子点有了明显的提高,说明通过低分子量壳聚糖的包裹可以明显提高纳米材料的生物相容性。因此,这类具有较好水溶性和生物相容性的荧光 AgInS2/LCSMS纳米复合材料可作为优良的生物荧光标记材料在生物医学检验、细胞以及活体成像研究中有广泛的应用前景。
선용천연다당중유일적감성다당———각취당작위은정제화포과제,성공합성료수용성적AgInS2양자점/저분자량각취당납미복합재료( AgInS2/LCSMS)。이용투사전자현미경( TEM)、FT-IR부리협홍외광보의、자외흡수광보、형광분광광도계등표정수단대납미복합재료적형모、화학조성급광학성질진행료연구。결과표명,AgInS2/LCSMS납미복합재료적립경약위5~6 nm,재수상중잉구유교은정적발광。지후,대AgInS2/LCSMS납미복합재료적생물상용성진행료연구,대비AgInS2/LCSMS납미복합재료여AgInS2양자점적세포활성측시결과발현,AgInS2/LCSMS납미복합재료적세포활성비AgInS2양자점유료명현적제고,설명통과저분자량각취당적포과가이명현제고납미재료적생물상용성。인차,저류구유교호수용성화생물상용성적형광 AgInS2/LCSMS납미복합재료가작위우량적생물형광표기재료재생물의학검험、세포이급활체성상연구중유엄범적응용전경。
The water-soluble AgInS2 quantum dots/low molecular weight chitosan nanocomposites (AgInS2/LCSMS) were successfully synthesized. Natural polysaccharide in alkaline polysaccharide———Chitosan was used as stabilizer and capped agent. The structure, chemical composition, and optical properties of AgInS2/LCSMS nanocomposites were studied by transmission electron microscopy ( TEM) , Fourier transform infrared spectroscopy (FT-IR), fluorescence microscope, fluorescence spectrophotome-ter, and UV-Vis absorption spectroscopy, etc. The results show that the particle diameter of AgInS2/LCSMS nanocomposites is about 5-6 nm, and they are still have a relatively stable luminescence in the water phase. In addition, we studied the biological compatibility of the AgInS2/LCSMS nanocomposites. Compared with pure AgInS2 quantum dots, the cell activity of AgInS2/LCSMS nanocomposites has an ob-vious improvement. It shows that low molecular weight chitosan wrapping can obviously improve the bio-compatibility of the nanometer materials. Therefore, this kind of AgInS2/LCSMS fluorescent nanocompos-ites with good water solubility and biological compatibility show significant value for potential applications in biomedicine test, biolabel, cell and in vivo imaging studies.