CAJ | 학술논문

目的：发展一种适宜在玻璃－聚二甲基硅氧烷（ PDMS）微流控芯片微通道内建立温度梯度场的新方法，并验证其在细胞热生物效应研究中的适用性。方法微通道内温度梯度场的建立和控制采用外围铟锡氧化物（ ITO）加热器和埋入PDMS芯片的加热微丝；有限元数值分析和温度依赖性荧光染料罗丹明B对微通道内建立的温度梯度场进行表征；以细胞存活率为指标，在微通道内考察人前列腺肿瘤细胞T24的热生物学效应。结果有限元数值分析结果显示，该方法在沿微通道长度方向成功建立温度梯度场，其分布范围受ITO加热器控制，温度梯度场梯度变化受加热微丝控制；罗丹明B实验测量结果与有限元数值分析结果相吻合；T24肿瘤细胞热生物学效应研究显示，微通道细胞存活率随着区域温度值上升而下降。结论该研究开发的在玻璃-PDMS微流控芯片微通道内构建温度梯度场方法简单且易于实现，未来可应用于微流控芯片上细胞热生物学效应的并行化研究。
목적：발전일충괄의재파리－취이갑기규양완（ PDMS）미류공심편미통도내건립온도제도장적신방법，병험증기재세포열생물효응연구중적괄용성。방법미통도내온도제도장적건립화공제채용외위인석양화물（ ITO）가열기화매입PDMS심편적가열미사；유한원수치분석화온도의뢰성형광염료라단명B대미통도내건립적온도제도장진행표정；이세포존활솔위지표，재미통도내고찰인전렬선종류세포T24적열생물학효응。결과유한원수치분석결과현시，해방법재연미통도장도방향성공건립온도제도장，기분포범위수ITO가열기공제，온도제도장제도변화수가열미사공제；라단명B실험측량결과여유한원수치분석결과상문합；T24종류세포열생물학효응연구현시，미통도세포존활솔수착구역온도치상승이하강。결론해연구개발적재파리-PDMS미류공심편미통도내구건온도제도장방법간단차역우실현，미래가응용우미류공심편상세포열생물학효응적병행화연구。
Objective To develop a new method for establishing a temperature gradient field in the microchannel on a glass-polydimethylsiloxane ( PDMS ) microfluidic chip and to verify its applicability in the study of cellular thermal biological effect.Methods The establishment and control of the temperature gradient field in the microchannel were implemented by a peripheral indium tin oxide ( ITO) heater and a heating micro-wire embedded in the PDMS chip.The temperature gradient field established in the microchannel was represented by the finite element numerical analysis and temperature-dependent fluorescent dye rhodamine B.Finally, the thermal biological effect, which used cell survival rate of human prostate cancer cells T24 as an indicator, was investigated in the microchannel.Results The results of finite element numerical analysis proved that this method established a temperature gradient field along the length of the microchannel successfully.The distribution range of the temperature gradient field was controlled by the ITO heater, while the gradient of the temperature gradient field was controlled by the heating micro-wire.The measurement result of rhodamine B was identical with the result of the finite element numerical analysis.The thermal biological effect of T24 tumor cell research showed that the cell survival rate decreased with the rise of the regional temperature in the microchannel.Conclusion The method developed in this paper for establishing a temperature gradient field in the microchannel on a glass-PDMS microfluidic chip is simple and easy to implement, and it can be used for parallel study of the cellular thermal biological effect on the microfluidic chip in the future.