国际免疫学杂志
國際免疫學雜誌
국제면역학잡지
International Journal of Immunology
2015年
5期
436-439
,共4页
硫酸镁%重度子痫前期%Toll样受体4%基因表达
硫痠鎂%重度子癇前期%Toll樣受體4%基因錶達
류산미%중도자간전기%Toll양수체4%기인표체
Magnesium sulphate%Severe preeclampsia%Toll-like receptor 4%Gene expression
目的 分析硫酸镁对重度子痫前期(SP)产妇Toll样受体4(TLR4)表达的影响.方法 选择同期正常产妇90例为A组、采取硫酸镁治疗的SP产妇90例为B组以及未采取硫酸镁治疗的SP产妇90例为C组,均用RT-PCR与免疫组化(SP法)检测胎盘中TLR4 mRNA和TLR4蛋白的表达,用酶联接免疫吸附试验(FLISA)法检测产妇血清中TLR4蛋白的表达,对检查结果进行分组统计并分析.结果 C组TLR4 mRNA、TLR4蛋白阳性率和血清中TLR4蛋白水平均显著高于A组(t=3.358,P＜0.01),B组TLR4表达未降至正常水平,但TLR4 mRNA、TLR4蛋白阳性率和血清中TLR4蛋白水平均明显低于C组(t=2.023,P＜0.05).B组患者治疗后各项实验室检查指标、不良症状与体征均明显好转(x2=49.585,P＜0.01).结论 硫酸镁可能通过下调TLR4表达而起到有效的控制SP的作用.
目的 分析硫痠鎂對重度子癇前期(SP)產婦Toll樣受體4(TLR4)錶達的影響.方法 選擇同期正常產婦90例為A組、採取硫痠鎂治療的SP產婦90例為B組以及未採取硫痠鎂治療的SP產婦90例為C組,均用RT-PCR與免疫組化(SP法)檢測胎盤中TLR4 mRNA和TLR4蛋白的錶達,用酶聯接免疫吸附試驗(FLISA)法檢測產婦血清中TLR4蛋白的錶達,對檢查結果進行分組統計併分析.結果 C組TLR4 mRNA、TLR4蛋白暘性率和血清中TLR4蛋白水平均顯著高于A組(t=3.358,P＜0.01),B組TLR4錶達未降至正常水平,但TLR4 mRNA、TLR4蛋白暘性率和血清中TLR4蛋白水平均明顯低于C組(t=2.023,P＜0.05).B組患者治療後各項實驗室檢查指標、不良癥狀與體徵均明顯好轉(x2=49.585,P＜0.01).結論 硫痠鎂可能通過下調TLR4錶達而起到有效的控製SP的作用.
목적 분석류산미대중도자간전기(SP)산부Toll양수체4(TLR4)표체적영향.방법 선택동기정상산부90례위A조、채취류산미치료적SP산부90례위B조이급미채취류산미치료적SP산부90례위C조,균용RT-PCR여면역조화(SP법)검측태반중TLR4 mRNA화TLR4단백적표체,용매련접면역흡부시험(FLISA)법검측산부혈청중TLR4단백적표체,대검사결과진행분조통계병분석.결과 C조TLR4 mRNA、TLR4단백양성솔화혈청중TLR4단백수평균현저고우A조(t=3.358,P＜0.01),B조TLR4표체미강지정상수평,단TLR4 mRNA、TLR4단백양성솔화혈청중TLR4단백수평균명현저우C조(t=2.023,P＜0.05).B조환자치료후각항실험실검사지표、불량증상여체정균명현호전(x2=49.585,P＜0.01).결론 류산미가능통과하조TLR4표체이기도유효적공제SP적작용.
Objective To evaluate the effect of magnesium sulfate (MgSO4) on the expression of toll-like receptor 4 (TLR4) in the patients with severe preeclampsia (SP).Methods 90 normal parturients were designated as group A,90 parturients with SP treated by magnesium sulfate were designated as group B,and 90 parturients cases without the treatment by magnesium sulfate were designated as group C.TLR4 mRNA and protein in placenta were detected by RT-PCR and immunohistochemistry.TLR4 levels in serum were studied by enzyme-linked immunosorbent assay (ELISA).Results The expression of TLR4 mRNA,TLR4 protein in placenta and the levels of TLR4 in serum of Group group C were significantly higher than those of group A (t =3.358,P ＜0.01).The expression of TLR4 in the group B was not reduced to the normal range level,but TLR4 mRNA,the positive rate of TLR4 protein,and the levels of TLR4 in serum were significantly lower than those in the group C (t =2.023,P ＜ 0.05).After the treatment with magnesium sulfate,the laboratory examination,adverse symptoms and signs of the parturients in group B were significantly improved (x2 =49.585,P ＜ 0.01).Condusion Magnesium sulfate can control SP by down-regulateding the expression of TLR4.
