CAJ | 학술논문

本试验旨在研究不同剂型丁酸钠对脂多糖( LPS)应激肉鸡血清生化指标、抗氧化和抗炎功能的影响。饲养试验将1000羽1日龄岭南黄肉鸡随机分为5组,每组4个重复,每重复50羽。对照组饲喂基础饲粮,试验组在基础饲粮中分别添加500 mg/kg粉剂丁酸钠( NaB)及250、500、750 mg/kg包膜丁酸钠( CNaB),试验为期50 d。应激试验分别从对照组、NaB组以及生长性能最佳组(250 mg/kg CNaB组),每重复随机选6只鸡,按2×3因子试验设计,以应激种类(生理盐水和LPS)和饲粮类型(0、500 mg/kg NaB和250 mg/kg CNaB)作为主效因子,各组1/2鸡只于53、56和59 d腹腔注射大肠杆菌LPS(0.5 mg/kg BW),剩余鸡只则注射等量生理盐水,试验为期10 d。结果表明：1)与生理盐水注射组相比,LPS注射组显著降低了血清中葡萄糖和甘油三酯含量( P<0.05),显著提高了血清尿酸含量( P<0.05)。 LPS注射下,NaB组和CNaB组中血清葡萄糖含量均显著高于对照组( P<0.05)。2)与生理盐水注射组相比,LPS注射组显著或极显著降低了血清和肝脏中超氧化物歧化酶( SOD)和谷胱甘肽过氧化物酶( GSH-Px)活性( P<0.05或P<0.01),显著降低了血清过氧化氢酶(CAT)活性(P<0.05)；LPS注射下,NaB 组和CNaB组血清和肝脏SOD、CAT活性均高于对照组(P>0.05),丙二醛(MDA)含量低于对照组( P>0.05)。 LPS注射和饲粮添加丁酸钠对肝脏GSH-Px活性影响存在交互作用( P<0.05),且2种剂型作用效果相类似。3)与生理盐水注射组相比,LPS注射组极显著提高53和59 d血清铜蓝蛋白和白细胞介素-6( IL-6)含量( P<0.01)；LPS注射下,NaB组和CNaB组53 d血清铜蓝蛋白含量显著低于对照组(P<0.05)。由此可见,在 LPS应激条件下,丁酸钠能改善肉鸡营养代谢,维持机体抗氧化功能,增强抗炎功能,且500 mg/kg NaB和250 mg/kg CNaB (有效含量为75 mg/kg)作用效果基本一致。
본시험지재연구불동제형정산납대지다당( LPS)응격육계혈청생화지표、항양화화항염공능적영향。사양시험장1000우1일령령남황육계수궤분위5조,매조4개중복,매중복50우。대조조사위기출사량,시험조재기출사량중분별첨가500 mg/kg분제정산납( NaB)급250、500、750 mg/kg포막정산납( CNaB),시험위기50 d。응격시험분별종대조조、NaB조이급생장성능최가조(250 mg/kg CNaB조),매중복수궤선6지계,안2×3인자시험설계,이응격충류(생리염수화LPS)화사량류형(0、500 mg/kg NaB화250 mg/kg CNaB)작위주효인자,각조1/2계지우53、56화59 d복강주사대장간균LPS(0.5 mg/kg BW),잉여계지칙주사등량생리염수,시험위기10 d。결과표명：1)여생리염수주사조상비,LPS주사조현저강저료혈청중포도당화감유삼지함량( P<0.05),현저제고료혈청뇨산함량( P<0.05)。 LPS주사하,NaB조화CNaB조중혈청포도당함량균현저고우대조조( P<0.05)。2)여생리염수주사조상비,LPS주사조현저혹겁현저강저료혈청화간장중초양화물기화매( SOD)화곡광감태과양화물매( GSH-Px)활성( P<0.05혹P<0.01),현저강저료혈청과양화경매(CAT)활성(P<0.05)；LPS주사하,NaB 조화CNaB조혈청화간장SOD、CAT활성균고우대조조(P>0.05),병이철(MDA)함량저우대조조( P>0.05)。 LPS주사화사량첨가정산납대간장GSH-Px활성영향존재교호작용( P<0.05),차2충제형작용효과상유사。3)여생리염수주사조상비,LPS주사조겁현저제고53화59 d혈청동람단백화백세포개소-6( IL-6)함량( P<0.01)；LPS주사하,NaB조화CNaB조53 d혈청동람단백함량현저저우대조조(P<0.05)。유차가견,재 LPS응격조건하,정산납능개선육계영양대사,유지궤체항양화공능,증강항염공능,차500 mg/kg NaB화250 mg/kg CNaB (유효함량위75 mg/kg)작용효과기본일치。
The trial was conducted to study on the effects of different type of sodium butyrate on serum bio-chemical indices, antioxidant and anti-inflammatory function of broilers challenged with lipopolysaccharide ( LPS) . Feeding trial: a total of 1 000 one-day-old Lingnan yellow broilers were randomly allocated into 5 groups with 4 replicates in each group and 50 broilers per replicate. Broilers in control group were fed a basal diet, the others in experimental groups were fed the basal diets supplemented with 500 mg/kg powder sodium butyrate ( NaB) , and 250, 500 and 750 mg/kg coated sodium butyrate ( CNaB) , respectively. The LPS in-jection experiment was conducted followed a 50-day feeding trial, 72 fifty-day-old female broilers were select-ed randomly from control, NaB and 250 mg/kg CNaB group with the best growth performance, with 6 broilers per replicate. And they were allotted into a 2×3 factorial arrangement with challenge status (saline or LPS ex-posure) and feed diet (0, 500 mg/kg NaB or 250 mg/kg CNaB) as main factor for endotoxin exposure test. Half of broilers in each group were injected intraperitoneally with E.coli LPS (0.5 mg/kg body weight)at 53, 56 and 59 d of age, the remaining broilers were injected intraperitoneally with the same amount of saline as the LPS injected group. The trial lasted for 10 days. The results showed as follows: 1) compared with the saline injected group, the content of glucose and riglyceride in serum was significantly decreased in LPS injected group (P<0.05), but the serum uric acid content as significantly increased (P<0.05). The serum glucose content of NaB and CNaB groups injected with LPS was significantly higher than that of control group ( P<0.05) . 2) Compared with the saline injected group, the activity of superoxide dismutase ( SOD) and glutathi-one peroxidase ( GSH-Px) in serum and liver was significantly decreased in LPS injected group ( P<0. 05 or P<0.01) , the serum catalase ( CAT) activity was significantly decreased ( P<0.05) . The activity of SOD and CAT in serum and liver of NaB and CNaB groups injected with LPS was higher than that of control group ( P>0.05), but the malonaldehyde content was lower than that of control group (P>0.05). There were a signifi-cant interaction between dietary sodium butyrate and LPS injected on liver GSH-Px activity ( P<0.05) , and the two forms of sodium butyrate exerted an equal effect on antioxidant enzyme activity. 3) Compared with the sa-line injected group, the content of ceruloplasmin and interleukin-6 in serum at the age of 53 and 59 days was significantly increased in LPS injected group ( P<0.05) . The serum ceruloplasmin content at the age of 53 days of NaB and CNaB groups injected with LPS was significantly lower than that of control group (P<0.05). In conclusion, sodium butyrate can improve the nutrient metabolism, maintain antioxidant function and promote anti-inflammation function, the effects on anti-stress of 500 mg/kg NaB are equal to those of 250 mg/kg CNaB which contains 75 mg/kg effective substance.