CAJ | 학술논문

目的：观察不同剂量高功率微波（ high power microwave， HPM）辐照对Wistar大鼠脑组织的损伤作用及氧化应激的影响，为建立微波辐射氧化损伤动物模型及抗辐射药物的评价提供依据。方法将80只健康成年雄性Wistar大鼠按析因设计随机分为16组，辐射平均功率密度分别为0、10、30及100 mW／cm2；取材时间分别为6 h、1、3及7 d。照射组照射时间为6 min。分别于各取材时间点活杀动物并取脑组织，采用比色法测量大鼠脑组织丙二醛（MDA）含量、蛋白质羰基含量和谷胱甘肽过氧化物酶（GSH-Px）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）活性。结果各辐射组MDA、蛋白质羰基含量基本随着辐射剂量的升高而升高，随取材时间的延长而降低。各辐射组SOD、GSH-px、CAT活性基本随辐射剂量的升高而降低，随取材时间的延长先降后升。结论微波辐射能引起大鼠脑组织的氧化应激反应，主要表现在大鼠脑组织脂质和蛋白质氧化损伤以及抗氧化物酶活性降低，且与辐射剂量、取材时间有一定的依赖性。
목적：관찰불동제량고공솔미파（ high power microwave， HPM）복조대Wistar대서뇌조직적손상작용급양화응격적영향，위건립미파복사양화손상동물모형급항복사약물적평개제공의거。방법장80지건강성년웅성Wistar대서안석인설계수궤분위16조，복사평균공솔밀도분별위0、10、30급100 mW／cm2；취재시간분별위6 h、1、3급7 d。조사조조사시간위6 min。분별우각취재시간점활살동물병취뇌조직，채용비색법측량대서뇌조직병이철（MDA）함량、단백질탄기함량화곡광감태과양화물매（GSH-Px）、초양화물기화매（SOD）、과양화경매（CAT）활성。결과각복사조MDA、단백질탄기함량기본수착복사제량적승고이승고，수취재시간적연장이강저。각복사조SOD、GSH-px、CAT활성기본수복사제량적승고이강저，수취재시간적연장선강후승。결론미파복사능인기대서뇌조직적양화응격반응，주요표현재대서뇌조직지질화단백질양화손상이급항양화물매활성강저，차여복사제량、취재시간유일정적의뢰성。
Objective To investigate the adverse effect of different doses of high power microwave(HPM) irradiation on oxidative stress in the brain of Wistar rats in order to contribute to establishing an animal model to evaluate protective agents which will be used for protection against microwave radiation.Methods Eighty male Wistar rats were randomly divided into 16 groups according to factor analysis.The average power density was 0,10,30 and 100 mW/cm2 and the sampling time was 6 h,1,3 and 7 d .The duration of exposure was 6 minutes for each radiation group.After exposure, the rats were sacrificed at each sampling time.Colorimetric method was used to measure the content of malondialdehyde(MDA) and protein carbonyl, the activity of GSH-px, SOD and CAT.Results The content of MDA and protein carbonyl of each radiation group was increased with the radiation dose, but decreased with the sampling time prolonged.The activity of superoxide dismutast(SOD),glutathion peroxidase(GSH-px) and catalase(CAT) in each radiation group was decreased with the radiation dose increased, and with the sampling time prolonged, but increased later.Conclusion Microwave radiation can cause oxidative stress in rats brain, as shown by the oxidative damage of lipid and protein and the decrease in the activity of antioxidant enzymes.Besides, the effect also depends on the radiation dose and sampling time.