CAJ | 학술논문

目的：探讨冻干保存对日本血吸虫病患者血清抗体检测的影响。方法将30份慢性日本血吸虫患者血清平均分为2份，其中1份冷冻干燥抽干为粉末，使用前用0.9%的生理盐水复溶到干燥前的体积，另1份无需处理，直接保存备用。用间接血凝法（indirect hemagglutination assay, IHA）、酶联免疫吸附实验（enzyme linked immunosorbent assays, ELISA ）、胶体染料试纸条法（dipstick dye immunoassay, DDIA）3种方法检测冻干复溶组和未处理组血清中日本血吸虫特异抗体，比较分析冻干复溶组与未处理组及3种检测方法阳性率的差异。结果未处理组血清经过IHA、ELISA和DDIA法检测，阳性率均为100%。冻干复溶组血清经3种方法检测，ELISA 法阳性率最高100%（30/30），其次为胶体染料法83.3%（25/30）和IHA法80.0%（24/30）。经统计学分析，未处理组及处理组血吸虫病患者血清抗体阳性检出率不存在统计学意义差异，3种免疫学方法检测未处理组及处理组血清抗体阳性率也不存在统计学意义差异。结论冻干复溶对日本血吸虫患者血清抗体检测影响较小，冷冻干燥保存方法或可成为日本血吸虫患者血清长期保存的有效方法之一。
목적：탐토동간보존대일본혈흡충병환자혈청항체검측적영향。방법장30빈만성일본혈흡충환자혈청평균분위2빈，기중1빈냉동간조추간위분말，사용전용0.9%적생리염수복용도간조전적체적，령1빈무수처리，직접보존비용。용간접혈응법（indirect hemagglutination assay, IHA）、매련면역흡부실험（enzyme linked immunosorbent assays, ELISA ）、효체염료시지조법（dipstick dye immunoassay, DDIA）3충방법검측동간복용조화미처리조혈청중일본혈흡충특이항체，비교분석동간복용조여미처리조급3충검측방법양성솔적차이。결과미처리조혈청경과IHA、ELISA화DDIA법검측，양성솔균위100%。동간복용조혈청경3충방법검측，ELISA 법양성솔최고100%（30/30），기차위효체염료법83.3%（25/30）화IHA법80.0%（24/30）。경통계학분석，미처리조급처리조혈흡충병환자혈청항체양성검출솔불존재통계학의의차이，3충면역학방법검측미처리조급처리조혈청항체양성솔야불존재통계학의의차이。결론동간복용대일본혈흡충환자혈청항체검측영향교소，냉동간조보존방법혹가성위일본혈흡충환자혈청장기보존적유효방법지일。
Objective To discuss the influence of lyophilization and redissolution to detection on serum antibodies of schistosomasis japonica patients. Methods Thirty sera samples of chronic schistosomasis japonica patients were used in this study, Each serum was divided into 2 equal parts, one part of the sera was freeze dried as powder, the sera were redissoluted to the same volume with 0.9% normal saline at room tem-perature, the other part was without treatment and directly saved for further use. Serum samples of the untreat-ed group and lyophilization and redissolution group were all detected by indirect hemagglutination assay (IHA), enzyme linked immunosorbent assays (ELISA) and dipstick dye immunoassay (DDIA) methods for specific antibody of Schistosoma japonicum. The differences of positive rate between the untreated group and lyophilization and redissolution group and among the three detecting methods were statistically analyzed. Re-sults The positive rates of the untreated group detected by IHA, ELISA and DDIA were all 100%. The posi-tive rate of the lyophilization and redissolution group detected by ELISA was 100% (30/30), by DDIA method was 83.3%(25/30) and by IHA method was 80%(24/30). There was no significant difference between the un-treated group and lyophilization and redissolution group and among the three detecting methods. ConclusionThe influence of lyophilization and redissolution to detection on serum antibodies of schistosomasis japonica patients was rather small, so the freeze drying preservation method could be used for long term preservation of sera samples of schistosomasis japonica patients.