南京林业大学学报(自然科学版)
南京林業大學學報(自然科學版)
남경임업대학학보(자연과학판)
Journal of Nanjing Forestry University(Natural Science Edition)
2015年
5期
145-149
,共5页
李寒%李鑫%徐勇%陈牧%余世袁%勇强
李寒%李鑫%徐勇%陳牧%餘世袁%勇彊
리한%리흠%서용%진목%여세원%용강
竹加工剩余物%低聚木糖%乙醇%内切木聚糖酶%纤维素酶
竹加工剩餘物%低聚木糖%乙醇%內切木聚糖酶%纖維素酶
죽가공잉여물%저취목당%을순%내절목취당매%섬유소매
bamboo processing residues%xylo?oligosaccharides%ethanol%endo xylanase%cellulase
以竹加工废弃物中纤维素、木聚糖组分高附加值利用为目的,采用内切木聚糖酶选择性降解竹屑木聚糖,制备高附加值产品低聚木糖,竹屑制备低聚木糖的废渣经糖化、发酵制备乙醇. 采用7%的NaOH提取竹屑木聚糖1 h,木聚糖提取率为57.81%,竹屑木聚糖再在25 U/g内切木聚糖酶定向水解下,低聚木糖得率为39.49%,低聚木糖对青春双歧杆菌有良好的增殖作用. 竹屑木聚糖抽提剩余物在25 U/g纤维素酶和4 U/g β-葡萄糖苷酶条件下水解72 h,纤维素水解得率64.05%. 121.20 g/L纤维素水解糖液经酿酒酵母NLH13发酵40 h,糖利用率和乙醇得率分别为98. 80%和92. 95%. 研究结果可为竹加工废弃物的综合利用提供新的途径.
以竹加工廢棄物中纖維素、木聚糖組分高附加值利用為目的,採用內切木聚糖酶選擇性降解竹屑木聚糖,製備高附加值產品低聚木糖,竹屑製備低聚木糖的廢渣經糖化、髮酵製備乙醇. 採用7%的NaOH提取竹屑木聚糖1 h,木聚糖提取率為57.81%,竹屑木聚糖再在25 U/g內切木聚糖酶定嚮水解下,低聚木糖得率為39.49%,低聚木糖對青春雙歧桿菌有良好的增殖作用. 竹屑木聚糖抽提剩餘物在25 U/g纖維素酶和4 U/g β-葡萄糖苷酶條件下水解72 h,纖維素水解得率64.05%. 121.20 g/L纖維素水解糖液經釀酒酵母NLH13髮酵40 h,糖利用率和乙醇得率分彆為98. 80%和92. 95%. 研究結果可為竹加工廢棄物的綜閤利用提供新的途徑.
이죽가공폐기물중섬유소、목취당조분고부가치이용위목적,채용내절목취당매선택성강해죽설목취당,제비고부가치산품저취목당,죽설제비저취목당적폐사경당화、발효제비을순. 채용7%적NaOH제취죽설목취당1 h,목취당제취솔위57.81%,죽설목취당재재25 U/g내절목취당매정향수해하,저취목당득솔위39.49%,저취목당대청춘쌍기간균유량호적증식작용. 죽설목취당추제잉여물재25 U/g섬유소매화4 U/g β-포도당감매조건하수해72 h,섬유소수해득솔64.05%. 121.20 g/L섬유소수해당액경양주효모NLH13발효40 h,당이용솔화을순득솔분별위98. 80%화92. 95%. 연구결과가위죽가공폐기물적종합이용제공신적도경.
Xylo?oligosaccharides and ethanol preparation from bamboo processing residues were investigated. The bamboo xylan was selectively degraded by endo?xylanase to produce xylo?oligosaccharides. Xylan extraction residue was used for ethanol production through saccharification and fermentation processes. The xylan of bamboo processing residues was extracted with 7% NaOH solution for 1 h, the xylan extraction yield was 57.81%. The xylan was hydrolyzed by endo?xylanase with the dosage of 25 U/g xylan for 12 h, the xylo?oligosaccharides yield was 39.49%, and the xylo?oligosac?charides have good effect on the proliferation of Bifidobacterium adolescentis. The xylan extraction residue was hydrolyzed by cellulase and β?glucosidase with the dosage of 25 U/g and 4 U/g for 72 h, 64. 05% hydrolysis yield was obtained. The hydrolyzate containing 121.20 g/L glucose was fermented by Saccharomyces cerevisiae NLH13 for 40 h, the glucose consumption and ethanol yield were 98.80% and 92.95%, respectively.