南京林业大学学报(自然科学版)
南京林業大學學報(自然科學版)
남경임업대학학보(자연과학판)
Journal of Nanjing Forestry University(Natural Science Edition)
2015年
5期
52-58
,共7页
李彦%周晓东%方升佐%邓波%尚旭岚%杨万霞
李彥%週曉東%方升佐%鄧波%尚旭嵐%楊萬霞
리언%주효동%방승좌%산파%상욱람%양만하
青钱柳%光照强度%种源%季节动态变化%三萜类化合物
青錢柳%光照彊度%種源%季節動態變化%三萜類化閤物
청전류%광조강도%충원%계절동태변화%삼첩류화합물
Cyclocarya paliurus%illumination intensity%provenance%seasonal variation%triterpenoids
以青钱柳二根一干苗为研究对象,采用裂区设计研究了3种光照条件(全光照、50%全光照、15%全光照)和3个种源(五峰、沅陵、沐川)对青钱柳叶中总三萜、阿江榄仁酸和青钱柳甙Ⅰ含量的影响,探讨了青钱柳叶中三萜类化合物含量的季节性动态变化. 结果表明:以10月份作为青钱柳叶收获季节,不同处理青钱柳叶中三萜类化合物含量及产量存在显著差异. 总三萜含量以沅陵种源+全光照处理最高,为42.03 mg/g;阿江榄仁酸含量以沐川种源+15%全光照处理最高,为4. 42 mg/g;而青钱柳甙Ⅰ含量则以沐川种源+全光照处理最高,为2. 52 mg/g. 总三萜、青钱柳甙Ⅰ产量均以沐川种源+全光照处理最高,分别为2 907. 2 mg/株、264.4 mg/株;阿江榄仁酸产量以沅陵种源+50%全光照处理最高,为208.6 mg/株. 三萜类化合物含量的季节动态显示,其最高值均出现在9月. 青钱柳叶三萜类化合物含量与种源、光照强度以及叶片采摘时间密切相关,研究结果为青钱柳药用林的定向培育提供了支持.
以青錢柳二根一榦苗為研究對象,採用裂區設計研究瞭3種光照條件(全光照、50%全光照、15%全光照)和3箇種源(五峰、沅陵、沐川)對青錢柳葉中總三萜、阿江欖仁痠和青錢柳甙Ⅰ含量的影響,探討瞭青錢柳葉中三萜類化閤物含量的季節性動態變化. 結果錶明:以10月份作為青錢柳葉收穫季節,不同處理青錢柳葉中三萜類化閤物含量及產量存在顯著差異. 總三萜含量以沅陵種源+全光照處理最高,為42.03 mg/g;阿江欖仁痠含量以沐川種源+15%全光照處理最高,為4. 42 mg/g;而青錢柳甙Ⅰ含量則以沐川種源+全光照處理最高,為2. 52 mg/g. 總三萜、青錢柳甙Ⅰ產量均以沐川種源+全光照處理最高,分彆為2 907. 2 mg/株、264.4 mg/株;阿江欖仁痠產量以沅陵種源+50%全光照處理最高,為208.6 mg/株. 三萜類化閤物含量的季節動態顯示,其最高值均齣現在9月. 青錢柳葉三萜類化閤物含量與種源、光照彊度以及葉片採摘時間密切相關,研究結果為青錢柳藥用林的定嚮培育提供瞭支持.
이청전류이근일간묘위연구대상,채용렬구설계연구료3충광조조건(전광조、50%전광조、15%전광조)화3개충원(오봉、원릉、목천)대청전류협중총삼첩、아강람인산화청전류대Ⅰ함량적영향,탐토료청전류협중삼첩류화합물함량적계절성동태변화. 결과표명:이10월빈작위청전류협수획계절,불동처리청전류협중삼첩류화합물함량급산량존재현저차이. 총삼첩함량이원릉충원+전광조처리최고,위42.03 mg/g;아강람인산함량이목천충원+15%전광조처리최고,위4. 42 mg/g;이청전류대Ⅰ함량칙이목천충원+전광조처리최고,위2. 52 mg/g. 총삼첩、청전류대Ⅰ산량균이목천충원+전광조처리최고,분별위2 907. 2 mg/주、264.4 mg/주;아강람인산산량이원릉충원+50%전광조처리최고,위208.6 mg/주. 삼첩류화합물함량적계절동태현시,기최고치균출현재9월. 청전류협삼첩류화합물함량여충원、광조강도이급협편채적시간밀절상관,연구결과위청전류약용림적정향배육제공료지지.
Based on the seedlings of Cyclocarya paliurus ( 1-year stem with 2 year root system) , split-split plot design with three C. paliurus provenances ( Wufeng, Yuanling and Muchuan) and three light conditions ( full sunlight, 50%full sunlight and 15% full sunlight) was adopted to compare the content variation of total triterpenoid, arjunolic acid and cyclocarioside I in the leaves of C. paliurus seedlings under different treatments. Meanwhile, the seasonal variation of triterpenoid contents in the leaves was also discussed. The results showed that there were significant differences in triter?penoid accumulation in leaves of C. paliurus seedlings among different treatments when October is defined as leaf harvest time. The highest content of total triterpenoids (42.03 mg/g) was obtained in the treatment of Yuanling provenance with full sunlight, while the greatest contents of arjunolic acid and cyclocarioside I were achieved under the treatments of Mu?chuan provenance with 15% of full sunlight and Muchuan provenance with full sunlight, reaching 4.42 mg/g and 2.52 mg/g, respectively. The greatest production of total triterpenoids and cyclocarioside I was obtained in the treatment of Muchuan provenance with full sunlight, reaching 2 907. 2 and 264.4 mg/plant respectively. However, the highest pro?duction of arjunolic acid was observed in the treatments of Yuanling provenance with 50% of full sunlight, hitting to 208. 6 mg/plant. The accumulation of triterpenoids in the leaves of Cyclocarya paliurus are closely related to provenance, illumination intensity and the harvest time of leaves. Results from this study would provide some important practical and theoretical bases for effectively managing the plantations of C. paliurus.