CAJ | 학술논문

腐皮镰刀菌( Fusarium solani)是铁核桃( Juglans sigllata Dode)根腐病的重要致病菌,绛红褐链霉菌YSSPG3 ( Streptomyces purpeofuscus)对其具有较强的拮抗作用. 为了监测该生防菌在根际土壤中的定殖动态及在其作用下病原菌( F. solani)的动态变化,以盆栽铁核桃为研究对象,研究了S. purpeofuscus对接种F. solani植株的生防效果,并用实时荧光定量PCR技术分别对生防菌 S. purpeofuscus和病原菌F. solani在铁核桃根际土壤中动态变化进行研究. 结果表明,S. purpeofuscus对病原菌F. solani有较为明显的生防效果. 监测结果表明:接种短期内S. purpeofuscus迅速生长繁殖,20 d其相对表达量达到最高值,随后呈下降趋势,40 d后趋于稳定;接种S. purpeo?fuscus后,F. solani的相对生长量显著下降,60 d时降低了11 500%,进一步表明S. purpeofuscus对F. solani生防效果显著,可以作为铁核桃根腐病的前景生防菌.
부피렴도균( Fusarium solani)시철핵도( Juglans sigllata Dode)근부병적중요치병균,강홍갈련매균YSSPG3 ( Streptomyces purpeofuscus)대기구유교강적길항작용. 위료감측해생방균재근제토양중적정식동태급재기작용하병원균( F. solani)적동태변화,이분재철핵도위연구대상,연구료S. purpeofuscus대접충F. solani식주적생방효과,병용실시형광정량PCR기술분별대생방균 S. purpeofuscus화병원균F. solani재철핵도근제토양중동태변화진행연구. 결과표명,S. purpeofuscus대병원균F. solani유교위명현적생방효과. 감측결과표명:접충단기내S. purpeofuscus신속생장번식,20 d기상대표체량체도최고치,수후정하강추세,40 d후추우은정;접충S. purpeo?fuscus후,F. solani적상대생장량현저하강,60 d시강저료11 500%,진일보표명S. purpeofuscus대F. solani생방효과현저,가이작위철핵도근부병적전경생방균.
Fusarium solani is an important pathogen on Juglans sigllata causing root rot, which can be strongly antago?nistic by the strain Streptomyces purpeofuscus YSSPG3. In order to monitor the colonization dynamics of S. purpeofuscus on rhizosphere soil and the dynamic variation of F. solani, the same infectious indices of J. sigllata were studied as the re?search objects. Based on inoculating S. purpeofuscus to the pathogenetic plants,the dynamic changes of S. purpeofuscus and F. solaniin rhizosphere soil was investigated by real?time fluorescence quantitative PCR, and the dynamic coloniza?tion detection system of S. purpeofuscus and the dynamic change system of fungus were established respectively. The re?sults showed that the strain S.purpeofuscus YSSPG3 could multiplied rapidly in a short time after inoculation, the strain peaked on the 20th day and then declined until the 40th day, finally, it kept stable. On the 60th day, the relatively ex?pression quantity of F. solani roped nearly 11 500%, which showed significantly biocontrol effects on F. solani. The study provided a reliable technical support on the real?time monitoring of plant diseases caused by F. solani, it also layed an important theoretical foundation on the colonization detection of S. purpeofuscus in rhizosphere.