CAJ | 학술논문

铜绿假单胞菌( Pseudomonas aeruginosa)产生的拮抗物质对撑×绿杂交竹梢枯病病原菌具有明显的抑制作用. 铜绿假单胞菌发酵液预处理后,采用三氯甲烷萃取得到活性粗提物,后经硅胶柱色谱分离及紫外洗脱检测,得到抑菌活性最强的黄色油状活性物质. 经核磁共振和红外光谱检测,得出该活性物质是一种有芳香环的吩嗪类化合物,化学式为C14 H10 O2 N2. 色素稳定性的研究表明,光照、温度对活性色素的稳定性影响甚微;低浓度的氧化剂和还原剂对活性色素的稳定性影响较小,高浓度对其影响较大;色素在偏中性或碱性条件下( pH7~8)活性最大,当pH≤3时,色素性质不稳定,抑菌活性几近消失;金属离子对色素稳定性有不同程度的影响,其中Na+、K+、Ba2+、Ca2+、Ag+对色素的影响较小,Mg2+、Zn2+、Ni2+、EDTA-Na2 对色素稳定性有较大的影响,而 Al3+、Cu2+、Fe3+、Pb2+对色素活性影响很大,色素几乎失去活性.
동록가단포균( Pseudomonas aeruginosa)산생적길항물질대탱×록잡교죽소고병병원균구유명현적억제작용. 동록가단포균발효액예처리후,채용삼록갑완췌취득도활성조제물,후경규효주색보분리급자외세탈검측,득도억균활성최강적황색유상활성물질. 경핵자공진화홍외광보검측,득출해활성물질시일충유방향배적분진류화합물,화학식위C14 H10 O2 N2. 색소은정성적연구표명,광조、온도대활성색소적은정성영향심미;저농도적양화제화환원제대활성색소적은정성영향교소,고농도대기영향교대;색소재편중성혹감성조건하( pH7~8)활성최대,당pH≤3시,색소성질불은정,억균활성궤근소실;금속리자대색소은정성유불동정도적영향,기중Na+、K+、Ba2+、Ca2+、Ag+대색소적영향교소,Mg2+、Zn2+、Ni2+、EDTA-Na2 대색소은정성유교대적영향,이 Al3+、Cu2+、Fe3+、Pb2+대색소활성영향흔대,색소궤호실거활성.
The antagonistic substance of Pseudomonas aeruginosa can effectively antagonize Arthrinium phaeospermum (Corda)M.B.Elli. The yellow oily substances with strong microbe-inhibiting activity were included through silica gel col?umn chromatography and UV elution after pretreatment of the filtrate of fermentation broth adopting trichloromethane ex?traction. By nuclear magnetic resonance and infrared spectrum detection, the compound was proved a phenazine pigment with aromatic rings, and with the chemical formula C14 H10 O2 N2 . Furthermore, the pigment stability test showed that illu?mination and temperature exerted slight effects on pigment activity. Light concentrate oxidizer and reducing agent exerted small effects on it, while the heavy concentrate was on contrary. The pigment activity was up to its max when pH was 7-8; when pH≤3, it was instability and almost with no bacteriostatic activity. Metallic ion could affect the pigment stabili?ty in varying degrees, Na+,K+,Ba2+,Ca2+ and Ag+ exerted a light effect on pigment' s stability, however, Al3+,Cu2+, Fe3+,Pb2+ exerted a heavy influence, which nearly caused it lost all activities.