CAJ | 학술논문

本试验旨在研究不同微生态制剂对蛋鸡机体超氧化物歧化酶( SOD)调节机制的影响。选择360只健康的海兰褐蛋鸡,随机分成5组,每组3个重复,每个重复24只。对照组(Ⅰ组)饲喂基础饲粮,试验组(Ⅱ、Ⅲ、Ⅳ、Ⅴ组)分别在基础饲粮中添加0.5%中药乳酸菌、0.25%乳酸菌制剂+0.25%中药红曲霉制剂、0.5%乳酸菌制剂和0.5%中药红曲霉制剂。试验期为79 d。结果表明：1)与对照组相比,试验组中蛋鸡血清总超氧化物歧化酶( T-SOD)活性显著提高( P<0.05),Ⅱ、Ⅲ和Ⅳ组蛋黄中T-SOD活性显著提高( P<0.05)。2)与对照组相比,Ⅱ组蛋鸡肝脏中SOD1 mRNA的表达量显著提高(P<0.05),Ⅱ、Ⅳ和Ⅴ组的蛋鸡肝脏中SOD2 mRNA表达量显著提高( P<0.05)。由此可见,饲粮中添加上述微生态制剂能调节蛋鸡机体SOD机制,以添加0.5%中药乳酸菌制剂效果最好。
본시험지재연구불동미생태제제대단계궤체초양화물기화매( SOD)조절궤제적영향。선택360지건강적해란갈단계,수궤분성5조,매조3개중복,매개중복24지。대조조(Ⅰ조)사위기출사량,시험조(Ⅱ、Ⅲ、Ⅳ、Ⅴ조)분별재기출사량중첨가0.5%중약유산균、0.25%유산균제제+0.25%중약홍곡매제제、0.5%유산균제제화0.5%중약홍곡매제제。시험기위79 d。결과표명：1)여대조조상비,시험조중단계혈청총초양화물기화매( T-SOD)활성현저제고( P<0.05),Ⅱ、Ⅲ화Ⅳ조단황중T-SOD활성현저제고( P<0.05)。2)여대조조상비,Ⅱ조단계간장중SOD1 mRNA적표체량현저제고(P<0.05),Ⅱ、Ⅳ화Ⅴ조적단계간장중SOD2 mRNA표체량현저제고( P<0.05)。유차가견,사량중첨가상술미생태제제능조절단계궤체SOD궤제,이첨가0.5%중약유산균제제효과최호。
This experiment was conducted to investigate the effects of different kinds of probiotics on superox-ide dismutase ( SOD) regulatory mechanism of laying hens. A total of 360 healthy Hyline laying hens were randomly divided into 5 groups with 3 replicates per group and 24 hens per replicate. The hens in control group ( group Ⅰ) were fed a basal diet, and the others in experimental groups ( groupsⅡ,Ⅲ,ⅣandⅤ) were fed the basal diets supplemented with 0. 5% preparation of lactic acid bacteria with traditional Chinese medicine, 0.25% lactic acid bacteria preparation mixed with 0.25% Monascus preparations with traditional Chinese medi-cine, 0.5% lactic acid bacteria preparation and 0.5% Monascus preparations with traditional Chinese medicine, respectively. The experiment lasted for 79 days. The results showed as follows: 1 ) compared with the groupⅠ, the serum total superoxide dismutase ( T-SOD) activity of experimental groups was significantly increased ( P<0.05) , the yolk T-SOD activity of groupsⅡ, Ⅲand Ⅳ was significantly increased ( P<0.05) . 2) Com-pared with the groupⅠ, the liver SOD1 mRNA expression of groupⅡwas significantly increased ( P<0.05) , the liver SOD2 mRNA expression of group Ⅱ, Ⅳ and Ⅴ was significantly increased (P<0.05). In conclu-sion, different kinds of probiotics can regulate the mechanism on SOD of laying hens, and diets supplemented with 0.5% preparation of lactic acid bacteria with traditional Chinese medicine has the best result in this experi-ment.