动物营养学报
動物營養學報
동물영양학보
Chinese Journal Of Animal Nutrition
2015年
10期
3033-3040
,共8页
王钰明%赵峰%廖睿%张莉%张虎%张宏福
王鈺明%趙峰%廖睿%張莉%張虎%張宏福
왕옥명%조봉%료예%장리%장호%장굉복
生长猪%消化酶活性%养分消化率
生長豬%消化酶活性%養分消化率
생장저%소화매활성%양분소화솔
growing pig%digestive enzyme activity%nutrient digestibility
本试验旨在探讨猪空肠消化液中主要消化酶的活性与饲粮养分消化率间的相关性,为饲粮养分消化影响因素的研究提供参考。试验分为2组,分别饲喂玉米-豆粕型饲粮和玉米-玉米干酒糟及其可溶物( DDGS)-米糠粕型饲粮,每组设16个重复。测定饲粮和粪样的总能和干物质、粗蛋白质含量及空肠液中主要消化酶的活性,然后对每种饲粮的养分消化率与猪空肠液中主要消化酶的活性进行简单相关分析和典型相关分析。结果表明:1)2种饲粮下,生长猪个体间4种主要消化酶活性的变异系数均在20%以上,而饲粮干物质、粗蛋白质和总能消化率的变异系数均在1.5%以内;2)猪空肠液中淀粉酶、胰蛋白酶和糜蛋白酶的活性与饲粮养分消化率间无显著相关性(|r|≤0.51;P>0.05),而脂肪酶活性与玉米-豆粕型饲粮养分消化率呈显著正相关(r≥0.56;P<0.05),与玉米-玉米 DDGS-米糠粕型饲粮干物质消化率呈显著负相关(r=-0.51;P<0.05);3)猪空肠液消化酶活性与饲粮养分消化率间无典型相关关系( P>0.05),因此,脂肪酶活性对饲粮养分消化率变异的贡献有限。综合以上结果,生长猪空肠液消化酶活性与饲粮养分消化率间无稳定的相关性。
本試驗旨在探討豬空腸消化液中主要消化酶的活性與飼糧養分消化率間的相關性,為飼糧養分消化影響因素的研究提供參攷。試驗分為2組,分彆飼餵玉米-豆粕型飼糧和玉米-玉米榦酒糟及其可溶物( DDGS)-米糠粕型飼糧,每組設16箇重複。測定飼糧和糞樣的總能和榦物質、粗蛋白質含量及空腸液中主要消化酶的活性,然後對每種飼糧的養分消化率與豬空腸液中主要消化酶的活性進行簡單相關分析和典型相關分析。結果錶明:1)2種飼糧下,生長豬箇體間4種主要消化酶活性的變異繫數均在20%以上,而飼糧榦物質、粗蛋白質和總能消化率的變異繫數均在1.5%以內;2)豬空腸液中澱粉酶、胰蛋白酶和糜蛋白酶的活性與飼糧養分消化率間無顯著相關性(|r|≤0.51;P>0.05),而脂肪酶活性與玉米-豆粕型飼糧養分消化率呈顯著正相關(r≥0.56;P<0.05),與玉米-玉米 DDGS-米糠粕型飼糧榦物質消化率呈顯著負相關(r=-0.51;P<0.05);3)豬空腸液消化酶活性與飼糧養分消化率間無典型相關關繫( P>0.05),因此,脂肪酶活性對飼糧養分消化率變異的貢獻有限。綜閤以上結果,生長豬空腸液消化酶活性與飼糧養分消化率間無穩定的相關性。
본시험지재탐토저공장소화액중주요소화매적활성여사량양분소화솔간적상관성,위사량양분소화영향인소적연구제공삼고。시험분위2조,분별사위옥미-두박형사량화옥미-옥미간주조급기가용물( DDGS)-미강박형사량,매조설16개중복。측정사량화분양적총능화간물질、조단백질함량급공장액중주요소화매적활성,연후대매충사량적양분소화솔여저공장액중주요소화매적활성진행간단상관분석화전형상관분석。결과표명:1)2충사량하,생장저개체간4충주요소화매활성적변이계수균재20%이상,이사량간물질、조단백질화총능소화솔적변이계수균재1.5%이내;2)저공장액중정분매、이단백매화미단백매적활성여사량양분소화솔간무현저상관성(|r|≤0.51;P>0.05),이지방매활성여옥미-두박형사량양분소화솔정현저정상관(r≥0.56;P<0.05),여옥미-옥미 DDGS-미강박형사량간물질소화솔정현저부상관(r=-0.51;P<0.05);3)저공장액소화매활성여사량양분소화솔간무전형상관관계( P>0.05),인차,지방매활성대사량양분소화솔변이적공헌유한。종합이상결과,생장저공장액소화매활성여사량양분소화솔간무은정적상관성。
This experiment was conducted to investigate the relationship between digestive enzyme activity of jejunal fluid and dietary nutrient digestibility in pigs to provide reference for the study of nutrient digestion. Six-teen replicates per group were used in 2 groups, which were fed corn-soybean meal diet and corn-corn dry dis-tillers dried grains with soluble ( DDGS)-rice bran diet, respectively. The contents of dry matter ( DM) , crude protein ( CP) and gross energy ( GE) in the diets and feces, and digestive enzyme activity of jejunal fluid were determined. The relationship between digestive enzyme activity of jejunal fluid and dietary nutrient digestibility were calculated with simple correlation analysis and canonical correlation analysis. The results showed as fol-lows:1) the coefficient of variation ( CV) of 4 digestive enzymes were over 20 % and CV of digestibility of DM, CP and GE were no more than 1.5 % in pigs fed both diets. 2) No significant correlation were observed between the nutrient digestibility of diets and activity of amylase, trypsin or chymotrypsin (|r|≤0. 51, P>0.05) . Positive correlations were observed between the nutrient digestibility and activity of lipase in corn-soy-bean meal diet ( r≥0.56, P<0.05) . However, negative correlations were present between the DM digestibility and activity of lipase in corn-corn DDGS-rice bran diet ( r=-0.51, P<0.05) . 3) No significant canonical cor-relations were observed between the digestive enzyme activity of jejunal fluid and nutrient digestibility of diets in pigs ( P>0.05) , so the contribution of lipase activity to the variation of nutrient digestibility was little. In conclusion , there are no stable correlation between the nutrient digestibility of diets and digestive enzyme activi-ty of jejunal fluid in pigs.