CAJ | 학술논문

目的：探讨香烟烟雾（Cigarette smoke extract ，CSE）对清道夫受体A （scavenger receptor ， SR‐A）介导炎症相关性研究。方法制备CSE ，5％、10％和15％浓度CSE刺激小鼠肺泡巨噬细胞（M H‐S）6、12和24小时后，用实时荧光定量聚合酶链反应（PCR）技术检测SR‐A表达水平，酶联免疫吸附测定（ELISA）法测定炎症因子IL‐6、肿瘤坏死因子α（TNF‐α）表达水平。结果 CSE刺激巨噬细胞6小时、12小时和24小时后，与空白对照组相比，S R‐A表达水平升高，S R‐A表达水平随着研究的时间延长而逐渐升高（ P ＜0．01）。而相对应时间IL‐6和TNF‐α表达水平分别与空白对照相比，都显著升高且差异有统计学意义（ P ＜0．01）。通过直线相关分析统计得出SR‐A 表达水平与IL‐6表达水平呈正相关（ P ＜0．05），与TNF‐α表达水平亦呈正相关（ P ＜0．01）。结论香烟烟雾刺激肺泡巨噬细胞后SR‐A表达水平升高，且SR‐A表达与炎症因子IL‐6、TNF‐α表达成正相关，SR‐A可能参与介导CSE诱导的肺泡巨噬细胞的炎症反应。
목적：탐토향연연무（Cigarette smoke extract ，CSE）대청도부수체A （scavenger receptor ， SR‐A）개도염증상관성연구。방법제비CSE ，5％、10％화15％농도CSE자격소서폐포거서세포（M H‐S）6、12화24소시후，용실시형광정량취합매련반응（PCR）기술검측SR‐A표체수평，매련면역흡부측정（ELISA）법측정염증인자IL‐6、종류배사인자α（TNF‐α）표체수평。결과 CSE자격거서세포6소시、12소시화24소시후，여공백대조조상비，S R‐A표체수평승고，S R‐A표체수평수착연구적시간연장이축점승고（ P ＜0．01）。이상대응시간IL‐6화TNF‐α표체수평분별여공백대조상비，도현저승고차차이유통계학의의（ P ＜0．01）。통과직선상관분석통계득출SR‐A 표체수평여IL‐6표체수평정정상관（ P ＜0．05），여TNF‐α표체수평역정정상관（ P ＜0．01）。결론향연연무자격폐포거서세포후SR‐A표체수평승고，차SR‐A표체여염증인자IL‐6、TNF‐α표체성정상관，SR‐A가능삼여개도CSE유도적폐포거서세포적염증반응。
Objective Correlation between inflammation mediated by cigarette smoke extract (CSE) on the scavenger receptor was detected .Methods CSE was prepared and mouse alveolar macrophage (MH‐S) was stimulated by different concentration of CSE (5% 、10% 、15% ) and different time (6、12、24 hours) ,Real‐time polymerase chain reaction detected the SR‐A expression levels ,enzyme‐linked immunosorbent assay (ELISA) measured the level of IL‐6 and TNF‐α.Results CSE stimulated M H‐S for 6 hours ,12 hours and 24 hours ,the expression level of SR‐A was higher than that in control group and increased steadily with extending time ( P < 0 .01 ) .IL‐6 and TNF‐α expression levels , compared with the control ,were significantly increased ( P < 0 .01) .The expression level of SR‐A was positively correlated with concentration of IL‐6 and TNF‐α by linear correlation analysis ( P < 0 .05) . Conclusions There is an up‐regulated expression of SR‐A by CSE‐stimulated mouse alveolar macrophage ,and SR‐A expression level is positively correlated with levels of IL‐6 and TNF‐α.SR‐A might involved in mediating the alveolar macrophage inflammatory response induced by CSE .