国际呼吸杂志
國際呼吸雜誌
국제호흡잡지
International Journal of Respiration
2015年
18期
1401-1407
,共7页
饲鸽者肺%白介素10%单核苷酸多态性
飼鴿者肺%白介素10%單覈苷痠多態性
사합자폐%백개소10%단핵감산다태성
Pigeon breeder′s lung%Interleukin 10%Single nucleotide polymorphism
目的:探讨IL‐10基因单核苷酸多态性(single nucleotide polymorphism ,SNP)与新疆维吾尔族饲鸽者肺易感性的相关。方法根据纳入及排除标准总纳入92例,其中病例组32例,阴性对照组30例,正常对照组30例,对纳入者进行DNA提取,并在 UCSC数据库选取IL‐10的16个基因位点,采用Sequenom MassARRAY? SNP分型技术对选取的位点进行基因分型比较。结果新疆维吾尔族人群rs1800893、rs1800896存在GG、GA、AA三种基因型,rs1800894存在GG、GA基因型,rs1800872存在CC、CA、AA基因型,rs3024500、rs1800871存在CC、CT、T T基因型;其基因型及等位基因分布频率在三组比较差异均无统计学意义( P >0.05),rs117106652、rs142726516、rs3024489、rs145922845、rs5743623、rs5743625、rs146520891、rs149143243、rs150423829、rs41432052位点不存在基因多态性。结论 IL‐10基因多态性与维吾尔族PBL遗传易感性之间未发现统计学上的关联。
目的:探討IL‐10基因單覈苷痠多態性(single nucleotide polymorphism ,SNP)與新疆維吾爾族飼鴿者肺易感性的相關。方法根據納入及排除標準總納入92例,其中病例組32例,陰性對照組30例,正常對照組30例,對納入者進行DNA提取,併在 UCSC數據庫選取IL‐10的16箇基因位點,採用Sequenom MassARRAY? SNP分型技術對選取的位點進行基因分型比較。結果新疆維吾爾族人群rs1800893、rs1800896存在GG、GA、AA三種基因型,rs1800894存在GG、GA基因型,rs1800872存在CC、CA、AA基因型,rs3024500、rs1800871存在CC、CT、T T基因型;其基因型及等位基因分佈頻率在三組比較差異均無統計學意義( P >0.05),rs117106652、rs142726516、rs3024489、rs145922845、rs5743623、rs5743625、rs146520891、rs149143243、rs150423829、rs41432052位點不存在基因多態性。結論 IL‐10基因多態性與維吾爾族PBL遺傳易感性之間未髮現統計學上的關聯。
목적:탐토IL‐10기인단핵감산다태성(single nucleotide polymorphism ,SNP)여신강유오이족사합자폐역감성적상관。방법근거납입급배제표준총납입92례,기중병례조32례,음성대조조30례,정상대조조30례,대납입자진행DNA제취,병재 UCSC수거고선취IL‐10적16개기인위점,채용Sequenom MassARRAY? SNP분형기술대선취적위점진행기인분형비교。결과신강유오이족인군rs1800893、rs1800896존재GG、GA、AA삼충기인형,rs1800894존재GG、GA기인형,rs1800872존재CC、CA、AA기인형,rs3024500、rs1800871존재CC、CT、T T기인형;기기인형급등위기인분포빈솔재삼조비교차이균무통계학의의( P >0.05),rs117106652、rs142726516、rs3024489、rs145922845、rs5743623、rs5743625、rs146520891、rs149143243、rs150423829、rs41432052위점불존재기인다태성。결론 IL‐10기인다태성여유오이족PBL유전역감성지간미발현통계학상적관련。
Objective To investigate relationship between gene single interleukin 10 (IL‐10 ) nucleotide polymorphisms (Single nucleotide polymorphism ,SNP) and Xinjiang Uygur lung susceptibility to feed pigeons .Methods Based on inclusion and exclusion criteria ,92 total cases ,including 32 cases of patient group ,the negative control group of 30 patients ,30 cases of normal control group were enrolled . All the people were subjected to DNA extraction ,and 16 loci of IL‐10 was selected in the UCSC database , using the Sequenom MassARRAY? SNP genotyping technology sites selected for genotyping .Results GG ,GA ,AA three kinds genotype were existed in Xinjiang Uygur people rs1800893 ,rs180089 .GG ,GA genotype were existed in rs1800894 and CC ,CA ,AA genotype were existed in rs1800872 .CC ,CT ,TT genotype were existed in rs3024500 ,rs1800871 .Genotype and allele frequencies showed no significant difference among three groups ( P > 0 .05 ) ,rs117106652 ,rs142726516 ,rs3024489 ,rs145922845 , rs5743623 ,rs5743625 ,rs146520891 ,rs149143243 ,rs150423829 ,rs41432052 did not exist polymorphism sites .Conclusions No association between IL‐10 gene polymorphism and genetic susceptibility Uighur PBL is found in this study .