CAJ | 학술논문

目的：研究初治肺结核患者外周血树突状细胞（DC）表面成熟标志的表达、分泌细胞因子以及对T淋巴细胞的刺激作用。方法收集初治肺结核患者68例，其中菌阴组35例，菌阳组33例。收集健康体检者40例作为对照组。分离外周血单个核细胞，诱导其分化成DC。流式细胞术检测各组DC的表面标志CD83和CD86的表达率；MTT法检测DC促进同种异体T淋巴细胞的增殖能力；ELISA法检测DC培养上清中白细胞介素（IL）-12、IL-10、干扰素（INF）-γ的含量。结果初治肺结核患者与对照组相比，DC表面标志CD83和CD86的表达量均显著降低（P＜0.05），刺激淋巴细胞增殖的能力均显著降低（P＜0.05），培养上清中IL-12的含量均显著增高（P＜0.05），培养上清中IL-10、INF-γ的含量各组间差异无统计学意义（P＞0.05）。结论初治肺结核患者外周血DC的成熟度显著下降，刺激混合淋巴细胞增殖的能力也显著降低，分泌IL-12的能力增强。
목적：연구초치폐결핵환자외주혈수돌상세포（DC）표면성숙표지적표체、분비세포인자이급대T림파세포적자격작용。방법수집초치폐결핵환자68례，기중균음조35례，균양조33례。수집건강체검자40례작위대조조。분리외주혈단개핵세포，유도기분화성DC。류식세포술검측각조DC적표면표지CD83화CD86적표체솔；MTT법검측DC촉진동충이체T림파세포적증식능력；ELISA법검측DC배양상청중백세포개소（IL）-12、IL-10、간우소（INF）-γ적함량。결과초치폐결핵환자여대조조상비，DC표면표지CD83화CD86적표체량균현저강저（P＜0.05），자격림파세포증식적능력균현저강저（P＜0.05），배양상청중IL-12적함량균현저증고（P＜0.05），배양상청중IL-10、INF-γ적함량각조간차이무통계학의의（P＞0.05）。결론초치폐결핵환자외주혈DC적성숙도현저하강，자격혼합림파세포증식적능력야현저강저，분비IL-12적능력증강。
Objective To investigate expressions of surface maturation markers, secreting cytokines and the stimulat?ing effect on T lymphocytes in the dendritic cells (DC) from peripheral blood of tuberculosis (TB) patients after loading dose treatment. Methods TB patients who received initial treatment (n=68) were collected at the fifth hospital of Shijiazhuang from 2013 June to 2014 January. Base on clinical diagnosis and treatment guidelines of tuberculosis, they were divided into sputum smear-negative group (35 cases) and sputum smear-positive group(33 cases). Forty cases of healthy adult were se?lected as control group. Mononuclear cells were isolated from peripheral blood and were cultured in medium to differentiate into DCs. Expression levels of CD83 and CD86 on DCs were examined by flow cytometry. The proliferation of allogeneic mixed lymphocyte stimulated by DCs was dectected using MTT assay. Contents of IL-12, IL-10 and INF-γin the cultural supernatant of DCs and blood serum from TB patients were detected by ELISA. Results Compared with controls ,the ex?pressions of CD83 and CD86 on DCs in TB patients after loading dose treatment decreased obviously(P<0.05), and the ability to stimulate the proliferation of lymphocytes reduced evidently(P<0.05). What’s more, IL-12 level increased mark?edly(P<0.05)while IL-10 and INF-γlevels presented no significant difference among the three groups (P>0.05). Conclu?sion The expressions of maturation markers of DC cells of the peripheral blood in TB patients after initial treatment de?creased. The ability of stimulating mixed lymphocyte proliferation is also significantly reduced while secretion of IL-12 was enhanced.