CAJ | 학술논문

目的：探讨雷奈酸锶对尾悬吊导致的应力缺失性大鼠骨质疏松的防治效果。方法6月龄SD大鼠30只，随机均分为3组：正常对照组（A组）、尾悬吊组（B组）、雷奈酸锶干预组（C组）。B、C两组大鼠采用尾悬吊法制备应力缺失型骨质疏松大鼠模型，C组给予1 g/(kg·d）雷奈酸锶干预，4周后处死所有大鼠，取左侧股骨检测骨密度，取左侧胫骨制备非脱钙组织切片并行骨形态计量学检测，取右侧股骨和胫骨骨髓细胞体外培养并向成骨细胞诱导分化，取第4代细胞及血清检测骨钙素（OCN）的表达。结果 B组骨密度低于A组，C组高于B组（P＜0.05）。B组骨小梁体积（BV/TV）、骨小梁数量（Tb.N）、骨小梁厚度（Tb.Th）低于A、C组，破骨细胞数（Oc.N）、骨吸收长度比（Er. Pm）高于A组，C组骨形成率（BFR/BV）、矿化长度比（L.Pm）高于B组，Er.Pm、Oc.N低于B组（P＜0.05）。B、C组OCN mRNA表达水平高于A组，但血清中OCN水平B组低于A、C组（P＜0.05）。结论尾悬吊4周可造成大鼠骨丢失，雷奈酸锶可抑制其骨量丢失，作用机制可能与其通过上调OCN的表达促进骨形成有关。
목적：탐토뢰내산송대미현조도치적응력결실성대서골질소송적방치효과。방법6월령SD대서30지，수궤균분위3조：정상대조조（A조）、미현조조（B조）、뢰내산송간예조（C조）。B、C량조대서채용미현조법제비응력결실형골질소송대서모형，C조급여1 g/(kg·d）뢰내산송간예，4주후처사소유대서，취좌측고골검측골밀도，취좌측경골제비비탈개조직절편병행골형태계량학검측，취우측고골화경골골수세포체외배양병향성골세포유도분화，취제4대세포급혈청검측골개소（OCN）적표체。결과 B조골밀도저우A조，C조고우B조（P＜0.05）。B조골소량체적（BV/TV）、골소량수량（Tb.N）、골소량후도（Tb.Th）저우A、C조，파골세포수（Oc.N）、골흡수장도비（Er. Pm）고우A조，C조골형성솔（BFR/BV）、광화장도비（L.Pm）고우B조，Er.Pm、Oc.N저우B조（P＜0.05）。B、C조OCN mRNA표체수평고우A조，단혈청중OCN수평B조저우A、C조（P＜0.05）。결론미현조4주가조성대서골주실，뢰내산송가억제기골량주실，작용궤제가능여기통과상조OCN적표체촉진골형성유관。
Objective To investigate the preventive effect of Strontium ranelate on stress-absence induced osteoporo?sis in tail-suspended rat. Methods A total of 30 SD rats with average age of 6 month were randomly divided into 3 groups (n=10 in each group):Group A was normal control group while rats in group B and C were subjected to tail suspension test to establish stress absence models. Rats in group C were administered with Strontium ranelate [1 g/(kg·d)]. All rats were sacri?ficed 4 weeks later. Left femurs were harvested for bone mineral density (BMD) test and prepared for undecalcified tissue sec?tion and thereby bone histomorphometry assessment. Bone marrow from right femurs and tibias were cultured and induced to?wards osteogenic-differentiation. The expression levels of osteocalcin in the fourth-passage cultured bone marrow cells and in blood serum were detected separately. Results Rats in group B showed markedly decreased BMD comparing to those in group A and C(P<0.05). Trabecular volume (BV/TV), number (Tb.N) and thickness (Tb.Th) in group B were lower than those in group A and C;erosion percentage (Er.Pm) and osteoclast number (Oc.N) in group B and C were higher than those in group A;comparing to those in group B, bone formation rate (BFR/BV), labeled percentage (L.Pm), were higher in group C, coupled with decreased Er.Pm and Oc.N(P<0.05). mRNA expression levels of OCN in group B and C were higher than those of group A. But its level in plasma were lower in group B than those in group A and C(P<0.05). Conclusion Tail suspension could induce osteosporosis. Strontium ranelate prevent bone loss in stress-absence osteoporosis in rat induced by tail-suspension for 4 weeks, which might be partially through upregulating the expression of OCN, thereby promoting bone formation.