CAJ | 학술논문

为了探讨板栗愈伤组织对栗疫病的抗性机制,采用栗疫菌Cp-毒素处理板栗愈伤组织,分析了毒素对不同抗性的板栗品种愈伤组织病程相关蛋白、植物保卫素及游离脯氨酸含量的影响. 研究表明:在较低浓度毒素处理范围内( 5~100 μg/mL) ,板栗愈伤组织内的游离脯氨酸含量、植物保卫素含量、几丁质酶和β-1,3-葡聚糖酶活性与Cp-毒素有关,抗病品种酶活性升幅大于感病品种;当毒素质量浓度高于100 μg/mL后,植物保卫素含量以及几丁质酶和β-1,3-葡聚糖酶活性下降,感病品种降幅大于抗病品种,而游离脯氨酸含量在品种间变化不大. 说明在毒素低浓度处理下不同品种抗性物质含量都有所增加,证明了几丁质酶和β-1,3-葡聚糖酶、植物保卫素是板栗愈伤组织抵抗病原菌毒素的内在机制,而游离脯氨酸含量变化不是引起品种抗性差异的重要因子.
위료탐토판률유상조직대률역병적항성궤제,채용률역균Cp-독소처리판률유상조직,분석료독소대불동항성적판률품충유상조직병정상관단백、식물보위소급유리포안산함량적영향. 연구표명:재교저농도독소처리범위내( 5~100 μg/mL) ,판률유상조직내적유리포안산함량、식물보위소함량、궤정질매화β-1,3-포취당매활성여Cp-독소유관,항병품충매활성승폭대우감병품충;당독소질량농도고우100 μg/mL후,식물보위소함량이급궤정질매화β-1,3-포취당매활성하강,감병품충강폭대우항병품충,이유리포안산함량재품충간변화불대. 설명재독소저농도처리하불동품충항성물질함량도유소증가,증명료궤정질매화β-1,3-포취당매、식물보위소시판률유상조직저항병원균독소적내재궤제,이유리포안산함량변화불시인기품충항성차이적중요인자.
In order to clarify the resistance mechanism of different chestnut cultivars to Cp-toxin stress, effects of differ?ent concentrations of Cp-toxin from Cryphonectria parasitica on the activities of chitinase, β-1,3-glucanase, the con?tents of phytoalexin, free proline (Pro) of chestnut callus were analyzed.In this study, a significant correlation was ob?served between the treatment concentration and chestnut resistance. The results showed that the activities of enzymes in?creased notably when treated with the low concentrations of Cp-toxin( 5-100μg/mL) . When treated with the high con?centrations of Cp-toxin(>100 μg/mL),the activities of enzymes and the contents of phytoalexin decreased notably. These data suggested that resistant materials in different varieties increased significantly under the treatment with low concentration of Cp-toxin. This study also indicates that the chitinase,β-1,3-glucanase and phytoalexins were the in?herent mechanism for resistance of chestnut callus to toxin stress, and change of free proline content was not an important factor related to the resistance.