CAJ | 학술논문

目的：探讨荷丹片对载脂蛋白E基因敲除（APOE-/-）小鼠炎症因子、氧化应激因子的影响及对动脉粥样硬化（AS）的干预作用及机制。方法将50只APOE-/-小鼠随机均分为对照组、模型组、荷丹片低剂量组、荷丹片高剂量组及辛伐他汀组。对照组给予普通饲料，其余组给予高胆固醇饲料造模。同时用药组灌胃相应剂量药物，对照组和模型组给予等量生理盐水灌胃。造模12周后测定血清白细胞介素（IL）-1、IL-10、丙二醛（MDA）、超氧化物歧化酶（SOD）水平；RT-PCR测主动脉肿瘤坏死因子（TNF）-αmRNA表达。结果模型组较对照组IL-1、MDA、TNF-αmRNA升高，IL-10、SOD降低（P<0.01）；荷丹片低剂量组、荷丹片高剂量组、辛伐他汀组较模型组IL-1、MDA、TNF-αmRNA降低，IL-10、SOD升高（P<0.01）。结论荷丹片可通过抗炎、抗氧化应激作用干预AS的发生、发展。
목적：탐토하단편대재지단백E기인고제（APOE-/-）소서염증인자、양화응격인자적영향급대동맥죽양경화（AS）적간예작용급궤제。방법장50지APOE-/-소서수궤균분위대조조、모형조、하단편저제량조、하단편고제량조급신벌타정조。대조조급여보통사료，기여조급여고담고순사료조모。동시용약조관위상응제량약물，대조조화모형조급여등량생리염수관위。조모12주후측정혈청백세포개소（IL）-1、IL-10、병이철（MDA）、초양화물기화매（SOD）수평；RT-PCR측주동맥종류배사인자（TNF）-αmRNA표체。결과모형조교대조조IL-1、MDA、TNF-αmRNA승고，IL-10、SOD강저（P<0.01）；하단편저제량조、하단편고제량조、신벌타정조교모형조IL-1、MDA、TNF-αmRNA강저，IL-10、SOD승고（P<0.01）。결론하단편가통과항염、항양화응격작용간예AS적발생、발전。
Objective To observe the effects of Hedan tablet on cytokines and oxidation factors in APOE-/-mouse, and to explore its effect on atherosclerosis and to explore its behind mechanism. Methods APOE-/-mice (n=50) were randomly divided into control group, model group, low dose Hedan tablet treatment group, high dose Hedan tablet treatment group and simvastatin treatment group. Mice in control group were given normal feed while mice in other groups were fed with high cho?lesterol diet. Hedan or Simvastatin was administrated intra-gastrically while normal saline was given to model group in the same route. After 12 weeks, mice were sacrificed to observe the mRNA level of tumor necrosis factor-α(TNF-αmRNA) in aorta by RT-PCR. Mean while, serum levels of interleukin-1 (IL-1), interleukin-10 (IL-10), malonaldehyde (MDA) and su?peroxide dismutase (SOD) were determined in different groups. Results Compared with control group, TNF-αmRNA tran?scription level as well as serum levels of IL-1 and MDA significantly increase while serum levels of IL-10 and SOD de?creased remarkably in model group, (P<0.01). Compared with model group, mRNA levels of TNF-αas well as serum levels of IL-1 and MDA were significantly decreased while serum levels of IL-10, SOD were greatly increased in low dose and high dose Hedan tablet treatment groups as well as in simvastatin treatment group (P<0.01). Conclusion Hedan tablet inhibit the formation of atherosclerosis through its anti-oxidation role and anti-inflammation role.