四川农业大学学报
四川農業大學學報
사천농업대학학보
Journal of Sichuan Agricultural University
2015年
3期
251-257,264
,共8页
蚕豆%种子萌发%PEG%抗旱性
蠶豆%種子萌髮%PEG%抗旱性
잠두%충자맹발%PEG%항한성
faba bean%seed germination%PEG stress%drought resistance
【目的】利用 PEG-6000胁迫对8份蚕豆品种的种子萌发、幼苗发根力和抗旱能力进行研究,为蚕豆抗旱种质资源鉴定评价及品种选育提供理论依据。【方法】采用不同渗透势:-0.09、-0.24、-0.43、-0.66、-0.85、-1.20 MPa 的 PEG-6000溶液(质量分数为5%、10%、15%、20%、25%、30%)模拟干旱胁迫测定种子萌发指标,分析其变化规律。【结果】随着 PEG-6000质量分数的增加,蚕豆种子的发芽率、发芽势、发芽指数、胚根长、胚芽干重总体呈下降趋势,低浓度的 PEG 胁迫对部分蚕豆品种种子萌发有一定的促进作用;随着胁迫程度的加剧,蚕豆种子的发芽率、发芽势、发芽指数显著降低,胚芽和胚根的生长受到抑制。【结论】15% PEG-6000浓度为合适的模拟干旱胁迫条件,PEG-6000质量分数越大,对蚕豆种子萌发和幼苗发根力的抑制作用越强。经隶属函数法分析蚕豆种子萌发期抗旱性强弱依次为:青海13号>尕大豆>临蚕9号>马牙>青蚕15号>临蚕6号>青海12号>青蚕14号。
【目的】利用 PEG-6000脅迫對8份蠶豆品種的種子萌髮、幼苗髮根力和抗旱能力進行研究,為蠶豆抗旱種質資源鑒定評價及品種選育提供理論依據。【方法】採用不同滲透勢:-0.09、-0.24、-0.43、-0.66、-0.85、-1.20 MPa 的 PEG-6000溶液(質量分數為5%、10%、15%、20%、25%、30%)模擬榦旱脅迫測定種子萌髮指標,分析其變化規律。【結果】隨著 PEG-6000質量分數的增加,蠶豆種子的髮芽率、髮芽勢、髮芽指數、胚根長、胚芽榦重總體呈下降趨勢,低濃度的 PEG 脅迫對部分蠶豆品種種子萌髮有一定的促進作用;隨著脅迫程度的加劇,蠶豆種子的髮芽率、髮芽勢、髮芽指數顯著降低,胚芽和胚根的生長受到抑製。【結論】15% PEG-6000濃度為閤適的模擬榦旱脅迫條件,PEG-6000質量分數越大,對蠶豆種子萌髮和幼苗髮根力的抑製作用越彊。經隸屬函數法分析蠶豆種子萌髮期抗旱性彊弱依次為:青海13號>尕大豆>臨蠶9號>馬牙>青蠶15號>臨蠶6號>青海12號>青蠶14號。
【목적】이용 PEG-6000협박대8빈잠두품충적충자맹발、유묘발근력화항한능력진행연구,위잠두항한충질자원감정평개급품충선육제공이론의거。【방법】채용불동삼투세:-0.09、-0.24、-0.43、-0.66、-0.85、-1.20 MPa 적 PEG-6000용액(질량분수위5%、10%、15%、20%、25%、30%)모의간한협박측정충자맹발지표,분석기변화규률。【결과】수착 PEG-6000질량분수적증가,잠두충자적발아솔、발아세、발아지수、배근장、배아간중총체정하강추세,저농도적 PEG 협박대부분잠두품충충자맹발유일정적촉진작용;수착협박정도적가극,잠두충자적발아솔、발아세、발아지수현저강저,배아화배근적생장수도억제。【결론】15% PEG-6000농도위합괄적모의간한협박조건,PEG-6000질량분수월대,대잠두충자맹발화유묘발근력적억제작용월강。경대속함수법분석잠두충자맹발기항한성강약의차위:청해13호>소대두>림잠9호>마아>청잠15호>림잠6호>청해12호>청잠14호。
[Objective]This study was carried out to explore the effects of Polyethylene glycol (PEG-6000)stress on seed germination,seeding root ability and drought resistance condition of eight cultivars of fava bean.[Method]Different osmotic potential of PEG-6000 (-0.09,-0.24,-0.43,-0.66,-0.85 and -1.20 MPa)were used to simulate drought stress.The indicators of seed germination were measured and change rules were analyzed.[Results]With the increase of PEG-6000 concentration,the germination rate,germination vigor,germination index,growth of plumule and germ dry weight of the fava bean seeds decreased generally.Low concentration of PEG improved seed germination of some fava bean variety materials.As the drought intensified, seed germination rate,germination vigor and germination index decreased and the growth of plu-mule and radical were inhibited.[Conclusion]The suitable PEG-6000 concentration for drought resistance identification was 15%.The larger PEG-6000 concentration was,the more severely the seed germination and seeding root ability of fava bean were influenced.The comprehensive evalua-tion on drought resistance of fava bean according to subordination function method indicated that the drought resistance ability were ranked as Qinghai 13 > Gadadou > Lincan 9 > Maya >Qingcan 15 > Lincan 6 > Qinghai 12 > Qingcan 14.