CAJ | 학술논문

目的：探讨树突状细胞疫苗的免疫学效应和抑瘤机制。方法制备负载MB 49细胞粗提全抗原的DC疫苗；建立小鼠MB 49膀胱癌皮下移植瘤模型。造模后第7 d、14 d，疫苗组小鼠于右前腋皮下注射疫苗，PBS对照组相应注射PBS，正常组正常饲养。造模第21 d，流式细胞术检测小鼠脾CD 4+、CD 4+ CD 69+T细胞数；取分离后的T细胞培养48 h，取上清，ELISA法检测IFN-γ水平。应用免疫组化方法检测瘤组织内CD 4+、CD 8+细胞数。结果小鼠脾CD 4+、CD 4+CD 69+细胞数，DC疫苗组高于PBS对照组和正常组，P＜0.01，差异具有统计学意义。（其中1组P＜0.05）。T细胞培养上清IFN-γ水平，DC疫苗组高于PBS对照组和正常组，P＜0.01，差异具有统计学意义。瘤组织内CD 4+、CD 8+T细胞数，DC疫苗组高于PBS对照组，P＜0.01，差异具有统计学意义。结论 DC 2.4疫苗刺激T细胞活化和增殖，使脾内CD 4+、CD 4+CD 69+细胞数增加，IFN-γ分泌增多，同时趋化迁移至瘤组织的CD 4+、CD 8+T细胞数增多，使模型小鼠自身杀伤清除肿瘤细胞能力增强，从而抑制了模型小鼠肿瘤的生长。
목적：탐토수돌상세포역묘적면역학효응화억류궤제。방법제비부재MB 49세포조제전항원적DC역묘；건립소서MB 49방광암피하이식류모형。조모후제7 d、14 d，역묘조소서우우전액피하주사역묘，PBS대조조상응주사PBS，정상조정상사양。조모제21 d，류식세포술검측소서비CD 4+、CD 4+ CD 69+T세포수；취분리후적T세포배양48 h，취상청，ELISA법검측IFN-γ수평。응용면역조화방법검측류조직내CD 4+、CD 8+세포수。결과소서비CD 4+、CD 4+CD 69+세포수，DC역묘조고우PBS대조조화정상조，P＜0.01，차이구유통계학의의。（기중1조P＜0.05）。T세포배양상청IFN-γ수평，DC역묘조고우PBS대조조화정상조，P＜0.01，차이구유통계학의의。류조직내CD 4+、CD 8+T세포수，DC역묘조고우PBS대조조，P＜0.01，차이구유통계학의의。결론 DC 2.4역묘자격T세포활화화증식，사비내CD 4+、CD 4+CD 69+세포수증가，IFN-γ분비증다，동시추화천이지류조직적CD 4+、CD 8+T세포수증다，사모형소서자신살상청제종류세포능력증강，종이억제료모형소서종류적생장。
Objective To explore the immunological effects and mechanisms of dendritic cell vaccine.Methods DC vaccine against MB 49 cells were prepared by loading MB 49 cells, and the model was established in mice. After making the model, the 7 days, 14 days, the vaccine were injected in right anterior axillary of vaccine group mice, and the mice of PBS control group were injected with PBS, normal feeding to normal group.21 days after modeling, lfow cytometry was used to detect the number of CD 4+ and CD 4+CD 69+T cells in spleen of mice. The T cells were cultured for 48 hours, and IFN-γ was detected by ELISA. The number of CD 4+ and CD 8+cells in tumor tissues was detected by immunohistochemical method.Results Mouse spleen, CD 4+andCD 4+CD 69+ cells count, DC vaccine group was higher than the PBS control group and normal group,P<0.01, had difference statistically signiifcance. ( one of themP<0.05 ). The level of IFN-γof T cell culture supernatant , DC vaccine group was higher than that of PBS control group and the normal group,P<0.01, had difference statistically signiifcance. The number of CD 4+, CD 8+ T cells in tumor tissue, DC vaccine group was higher than that of PBS control group,P<0.01, had difference statistically significance. ConclusionDC vaccine stimulated T cell activation and proliferation, increased the number of CD 4+, CD 4+ CD 69+ cells in the spleen,increased IFN-γsecretion, and increased the number of CD 4+, CD 8+T cells in the tumor tissues. DC 2.4 vaccine can inhibit the growth of tumor cells in mice by killing the tumor cells.