CAJ | 학술논문

目的分离纯化围植入期超早孕妇女血清中免疫抑制物质——早孕因子(early pregnancy factor,EPF).方法挑选取超声波监测的排卵受精后1到8d内的山西太原妇女40名,抽取血液20-40mL,RIT检测EPF活性,对收集的400 mL胚胎植入期妇女血清,经过DEAE Sepharose离子交换层析,Con A Sepharose 4B亲和层析以及Heparin Sepharose CL-6B亲和层析进行了EPF的分离纯化.结果受精后1-8天内的妇女血中可检测到EPF.EPF可以被纯化为相对分子质量为18000的单一条带.结论胚胎围植入期超早孕妇女血中EPF蛋白质的分离和纯化,为EPF的研究,尤其是围植入期免疫抑制作用,避免了母亲的免疫系统对半移植物胚胎的排斥的研究提供了理论和应用价值.
목적 분리순화위식입기초조잉부녀혈청중면역억제물질——조잉인자(early pregnancy factor,EPF).방법 도선취초성파감측적배란수정후1도8d내적산서태원부녀40명,추취혈액20-40mL,RIT검측EPF활성,대수집적400 mL배태식입기부녀혈청,경과DEAE Sepharose리자교환층석,Con A Sepharose 4B친화층석이급Heparin Sepharose CL-6B친화층석진행료EPF적분리순화.결과 수정후1-8천내적부녀혈중가검측도EPF.EPF가이피순화위상대분자질량위18000적단일조대.결론 배태위식입기초조잉부녀혈중EPF단백질적분리화순화,위EPF적연구,우기시위식입기면역억제작용,피면료모친적면역계통대반이식물배태적배척적연구제공료이론화응용개치.
Objective To purify early pregnancy factor (EPF)from the sera of pregnant women with immunosuppressive conditions.Methods Forty women in Taiyuan,Shanxi,China were chosen to detect ovulation by ultrasonagraphy daily.20 to 40 mL of blood was collected after 1 to 8 days of fertilization.400 mL sera from these women were subjected to the detection of EPF activity by rosette inhibition test(RIT).EPT was purified by DEAE Sepharose ion-exchange codumn,Con A sepharose 4b and Heparin Sepharose CL-6B.Result EPF was detected in the blood of pregnant women at 1 to 8 day after fertilization.EPF was purified by chromatography with molecular weight of 18 000.Conclusion Purification of EPF in the stage of periimplantation will provide the valuable significance of theoretical and clinical usage for the research of maternal immunologic tolerance of the allogenic fetus.