CAJ | 학술논문

目的探讨丙戊酸钠对人视网膜细胞瘤Y79细胞凋亡及其机制研究.方法用噻唑蓝(MTT)法检测不同浓度的VPA(浓度分别为0、0.5、1.0、2.0、4.0、8.0 mmol/L)对人视网膜细胞瘤Y79细胞增殖影响;流式细胞仪(FCM)对人视网膜细胞瘤Y79细胞凋亡水平的影响;Western Blot检测Y79细胞中P53、Bax和Bcl-2的表达水平.结果 MTT结果显示不同浓度VPA对人视网膜细胞瘤Y79细胞均具有抑制其增殖作用,其中2.0 mmol/L VPA处理Y79细胞24h、48 h和72 h后细胞存活率分别为84.25％、76.41％和59.36％(F=40.18、35.74和42.65,P均＜0.05).FCM结果显示VPA诱导Y79细胞24 h的凋亡水平为26.59％(P ＜0.05).Western Blot结果显示Y79细胞中P53和Bax均显著上升,Bcl-2无显著变化.结论丙戊酸钠能抑制Y79细胞的增殖和诱导其凋亡的作用,其机制与激活P53和Bax有关.
목적 탐토병무산납대인시망막세포류Y79세포조망급기궤제연구.방법 용새서람(MTT)법검측불동농도적VPA(농도분별위0、0.5、1.0、2.0、4.0、8.0 mmol/L)대인시망막세포류Y79세포증식영향;류식세포의(FCM)대인시망막세포류Y79세포조망수평적영향;Western Blot검측Y79세포중P53、Bax화Bcl-2적표체수평.결과 MTT결과현시불동농도VPA대인시망막세포류Y79세포균구유억제기증식작용,기중2.0 mmol/L VPA처리Y79세포24h、48 h화72 h후세포존활솔분별위84.25％、76.41％화59.36％(F=40.18、35.74화42.65,P균＜0.05).FCM결과현시VPA유도Y79세포24 h적조망수평위26.59％(P ＜0.05).Western Blot결과현시Y79세포중P53화Bax균현저상승,Bcl-2무현저변화.결론 병무산납능억제Y79세포적증식화유도기조망적작용,기궤제여격활P53화Bax유관.
Objective To investigate the mechanism of Valproic Acid (VPA) on retinoblastoma Y79 cell' s proliferation and apoptosis.Method MTT was used to detect the proliferation of Y79 cell after treated with different concentration of VPA (0,0.5,1.0,2.0,4.0 and 8.0 mmol/L).FCM was used to analyze the apoptosis level of Y79 cell.Western blot was used to detect P53,Bax and Bcl-2 in Y79 cell.Results MTT results showed that VPA could inhibit retinoblastoma Y79 cell' s proliferation after treated with different concentration of VPA.The survival fraction of Y79 cell was 84.25 ％,76.41％ and 59.36％ after 24 h,48 h and 72 h incubated with VPA,respectively (P ＜0.05).The apoptosis level induced by VPA was 26.59％ 24 h later in Y79 cell.P53 and Bax were largely increased (P ＜ 0.05),while Bcl-2 has no significant change (P ＞ 0.05).Conclusion VPA could inhibit retinoblastoma Y79 cell' s proliferation and induce apoptosis,which is associated with activation of P53 and Bax.