天津医药
天津醫藥
천진의약
Tianjin Medical Journal
2015年
10期
1122-1124
,共3页
王欣%董明%陈兴隆%李光
王訢%董明%陳興隆%李光
왕흔%동명%진흥륭%리광
肺移植%细支气管炎,闭塞性%疾病模型,动物%小鼠,近交C57BL%小鼠,近交BALB C
肺移植%細支氣管炎,閉塞性%疾病模型,動物%小鼠,近交C57BL%小鼠,近交BALB C
폐이식%세지기관염,폐새성%질병모형,동물%소서,근교C57BL%소서,근교BALB C
lung transplantation%bronchiolitis obliterans%disease models,animal%mice,inbred C57BL%mice,inbred BALB/C
目的：探讨改良原位气管移植模型的手术方法以提高生存率，减少手术时间。方法清洁级C57BL/6（供体）和BALB/c（受体）小鼠各30只，依体质量相近配对并随机分为3组。采用“经口插管气道内支架”吻合，原位气管移植建立动物模型，并记录手术时间。术后观察实验动物生存期及一般状况，并分别于术后15、30及60 d各处死10只，取出气管，HE染色观察移植气管的组织病理变化，计算气管阻塞率。结果受体小鼠均存活至取气管时，手术时间：供体气管摘除(7.0±1.0) min，受体气管摘除(7.0±1.0) min，经口插入套管（1.0±0.5）min，供体气管吻合（20.0±4.0）min，手术总时间（43.0±10.0）min。移植后气管淋巴细胞及中性粒细胞浸润，气管阻塞率于15、30及60 d逐渐增加，符合闭塞性细支气管炎病理特点。结论经口插管气道内支架吻合法建立小鼠原位气管移植模型操作简便，手术技巧和设备要求低，手术成功率高。不需要专业的外科技巧及手术设备。
目的：探討改良原位氣管移植模型的手術方法以提高生存率，減少手術時間。方法清潔級C57BL/6（供體）和BALB/c（受體）小鼠各30隻，依體質量相近配對併隨機分為3組。採用“經口插管氣道內支架”吻閤，原位氣管移植建立動物模型，併記錄手術時間。術後觀察實驗動物生存期及一般狀況，併分彆于術後15、30及60 d各處死10隻，取齣氣管，HE染色觀察移植氣管的組織病理變化，計算氣管阻塞率。結果受體小鼠均存活至取氣管時，手術時間：供體氣管摘除(7.0±1.0) min，受體氣管摘除(7.0±1.0) min，經口插入套管（1.0±0.5）min，供體氣管吻閤（20.0±4.0）min，手術總時間（43.0±10.0）min。移植後氣管淋巴細胞及中性粒細胞浸潤，氣管阻塞率于15、30及60 d逐漸增加，符閤閉塞性細支氣管炎病理特點。結論經口插管氣道內支架吻閤法建立小鼠原位氣管移植模型操作簡便，手術技巧和設備要求低，手術成功率高。不需要專業的外科技巧及手術設備。
목적：탐토개량원위기관이식모형적수술방법이제고생존솔，감소수술시간。방법청길급C57BL/6（공체）화BALB/c（수체）소서각30지，의체질량상근배대병수궤분위3조。채용“경구삽관기도내지가”문합，원위기관이식건립동물모형，병기록수술시간。술후관찰실험동물생존기급일반상황，병분별우술후15、30급60 d각처사10지，취출기관，HE염색관찰이식기관적조직병리변화，계산기관조새솔。결과수체소서균존활지취기관시，수술시간：공체기관적제(7.0±1.0) min，수체기관적제(7.0±1.0) min，경구삽입투관（1.0±0.5）min，공체기관문합（20.0±4.0）min，수술총시간（43.0±10.0）min。이식후기관림파세포급중성립세포침윤，기관조새솔우15、30급60 d축점증가，부합폐새성세지기관염병리특점。결론경구삽관기도내지가문합법건립소서원위기관이식모형조작간편，수술기교화설비요구저，수술성공솔고。불수요전업적외과기교급수술설비。
Objective To explore a simple and effective way of establishing a mice orthotopic tracheal transplantation model in studying bronchiolitis obliterans to reduce operation time. Methods Tracheas from C57BL/6 mice (n=30) were im?planted into Balb/c mice (n=30). Grafts and hosts were matched according to body weight."Orotracheal intubation and endo-stent"was used for trachea anastomose and animal model was establish. Operating time was also noted. Grafts were harvest?ed on Days 15, 30 and 60 after transplantation, using HE staining to observe pathological changes of allograft. Trachea block?ing rate was also calculated. Results Until survival of trachea transplantation, the operative time of donor′s and the recipi?ent′tracheas resection were (7.0±1.0) min and (7.0±1.0) min respectively."Orotracheal intubation the endo-stent"establish?ment cost(1 ± 0.5)min. Trachea anastomose took(20.0 ± 4.0)min and the operation time last(43.0 ± 10.0)min. Conclusion The improvement of orthotopic tracheas transplantation using"Orotracheal intubation the endo-sten"for trachea anastomose is esay-operated and did required complicate skills and instruments with high successful rate. So it is an ideal and stable model in studying bronchiolitis obliterans.
